Product Class: Kit

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)

Product Introduction

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity
 
Catalog # Size Concentration
E7780S 96.0 reactions

Product Information

Description

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional dual index primer options are available (NEB #E7600). Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535). 


Dual Index Workflow
Dual Index Workflow

This product is related to the following categories:

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E7780S     -20    
      NEBNext Adaptor for Illumina E7601AVIAL -20 1 x 0.96 ml Not Applicable
      USER® Enzyme E7602AVIAL -20 1 x 0.288 ml Not Applicable
      NEBNext® i509 Primer E7781AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i510 Primer E7782AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i511 Primer E7783AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i512 Primer E7784AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i513 Primer E7785AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i514 Primer E7786AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i515 Primer E7787AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i516 Primer E7788AVIAL -20 1 x 0.06 ml 10 µM
      NEBNext® i713 Primer E7789AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i714 Primer E7790AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i715 Primer E7791AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i716 Primer E7792AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i717 Primer E7793AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i718 Primer E7794AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i719 Primer E7795AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i720 Primer E7796AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i721 Primer E7797AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i722 Primer E7798AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i723 Primer E7799AVIAL -20 1 x 0.04 ml 10 µM
      NEBNext® i724 Primer E7800AVIAL -20 1 x 0.04 ml 10 µM

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) (E7780)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Where can I find the sample sheets for these Multiplex Oligos?
  2. Are NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) supplied with library prep reagents?
  3. Can I use NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) for library preparation from DNA and RNA samples?
  4. How many libraries can I prepare with the NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)?
  5. For pooling <96 samples, what combination of indices should I use?
  6. Do I need to spike in custom sequencing primers when sequencing libraries are made with NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) on Illumina sequencing instruments?
  7. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
  8. Is the USER® Enzyme in the NEBNext Oligos kits identical in concentration to the standalone product?
  9. How do I use the NEB provided Sample Sheet for my specific instrument?
  10. Are dual indexed libraries compatible with single end sequencing?
  11. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In