NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
E7370L |
NEBNext Ultra DNA Library Prep Kit for Illumina |
96 rxns | - | Unavailable in your region |
NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
The small size of this product was discontinued on December 15, 2021. If you would like to purchase a small size, an alternative is NEB #E7645S.
Product Introduction
This original version of the Ultra DNA Library Prep Kit enables library preparation from 5 ng – 1 µg of input DNA in a streamlined workflow. However, we now recommend use of the more recent NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645), which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 µg).Catalog # | Size | Concentration |
---|---|---|
E7370S | 24.0 reactions | |
E7370L | 96.0 reactions |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
The NEBNext® Ultra™ DNA Library Prep Kit for Illumina® contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 5 ng – 1 µg input DNA, in a streamlined workflow. Please note that adaptors and primers are not included in the kit and are available separately.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page- This product is related to the following categories:
Kit Components
Kit Components
The following reagents are supplied with this product:
Properties & Usage
Materials Required but not Supplied
- 80% Ethanol (freshly prepared)
- Nuclease-free Water
- 0.1X TE, pH 8.0
- 10 mM Tris-HCl, pH 7.5–8.0 (required for DNA input <100 ng)
- 5 M NaCl (required for DNA input <100 ng)
- DNA LoBind Tubes (Eppendorf #022431021)
- AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
- NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7710, #E7730, #E6609, or #E7600)
- Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
- PCR Machine
Features
- Input amounts as low as 5ng
- Fast workflow (<3 hours), with minimal hands-on time
- Robust, reliable performance
- Ultra high fidelity amplification with minimized GC bias
Protocols, Manuals & Usage
Protocols
Manuals
FAQs & Troubleshooting
FAQs
- Can AMPure XP beads used for clean-up steps be left in the PCR reactions?
- For what type of sample prep/library construction can I use the NEBNext Ultra DNA Library Prep kit for Illumina for?
- What type and how much starting material do I need to use when preparing libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina?
- Is size selection required and what is the recommended method?
- Can I use the NEBNext Ultra DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the Roche 454 GS FLX & SOLiD 5500?
- Can I use the NEBNext Ultra DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the ABI SOLiD 4 instrument or on the Ion Torrent PGM?
- Can NEB provide any of the reagents for the cluster generation step or the actual sequencing run?
- How do I know the concentration of my index primers?
- Which NEBNext Oligos can be used with this library prep kit?
- Can I use this NEBNext kit with adaptors and primers from other vendors than NEB?
- Are these reagents available in a customized format, larger format or 96 well formats?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- E7370S_v1_10009574
- E7370L_v1_10012611
- E7370L_v1_10016485
- E7370S_v1_10016491
- E7370L_v1_10021060
- E7370L_v1_10022754
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- E7370S_v1_10026478
- E7370L_v1_10029376
- E7370S_v1_10020440
- E7370L_v1_10020446
- E7370S_v1_10035722
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- E7370S_v1_10043241
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- E7370L_v1_10033338
- E7370S_v1_10049969
- E7370L_v1_10051221
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- E7370S_v1_10054513
- E7370L_v1_10044394
- E7370L_v1_10060078
- E7370L_v1_10064024
- E7370S_v1_10044386
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- E7370S_v1_10070241
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- E7370L_v1_10082547
- E7370L_v1_10059728
- E7370S_v1_10086565
- E7370L_v1_10087224
- E7370L_v1_10090526
- E7370L_v1_10094287
- E7370S_v1_10083108
- E7370L_v1_10103028
- E7370L_v1_10109362
- E7370L_v1_10114078
- E7370L_v1_10118050
- E7370S_v1_10120832
- E7370L_v1_10123887
- E7370L_v1_10106934
- E7370L_v1_10139817
- E7370L_v1_10141230
- E7370L_v1_10130684
- E7370L_v1_10157571
- E7370L_v1_10169933
- E7370L_v1_10186311
- E7370L_v1_10191207
- E7370L_v1_10193454
- E7370L_v1_10182310
- E7370L_v1_10227587
- E7370L_v1_10205229
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.NEBNext End Prep Enzyme Mix
NEBNext End Repair Reaction Buffer
Blunt/TA Ligase Master Mix
NEBNext® Ligation Enhancer
NEBNext Q5 Hot Start HiFi PCR Master Mix
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
For additional information or to inquire about commercial use, please contact busdev@neb.com.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.