The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7335S     -20       NEBNext Adaptor for Illumina E7337AVIAL -20 1 x 0.24 ml Not Applicable   USER® Enzyme E7338AVIAL -20 1 x 0.072 ml Not Applicable   NEBNext Universal PCR Primer for Illumina E6861AVIAL -20 1 x 0.12 ml 10 µM   NEBNext Index 1 Primer for Illumina E7311AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 2 Primer for Illumina E7312AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 3 Primer for Illumina E7313AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 4 Primer for Illumina E7314AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 5 Primer for Illumina E7315AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 6 Primer for Illumina E7316AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 7 Primer for Illumina E7317AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 8 Primer for Illumina E7318AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 9 Primer for Illumina E7319AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 10 Primer for Illumina E7320AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 11 Primer for Illumina E7321AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 12 Primer for Illumina E7322AVIAL -20 1 x 0.01 ml 10 µM
E7335L     -20       NEBNext Adaptor for Illumina E7337AAVIAL -20 1 x 0.96 ml Not Applicable   USER® Enzyme E7338AAVIAL -20 1 x 0.288 ml Not Applicable   NEBNext Universal PCR Primer for Illumina E6861AAVIAL -20 1 x 0.48 ml 10 µM   NEBNext Index 1 Primer for Illumina E7311AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 2 Primer for Illumina E7312AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 3 Primer for Illumina E7313AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 4 Primer for Illumina E7314AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 5 Primer for Illumina E7315AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 6 Primer for Illumina E7316AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 7 Primer for Illumina E7317AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 8 Primer for Illumina E7318AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 9 Primer for Illumina E7319AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 10 Primer for Illumina E7320AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 11 Primer for Illumina E7321AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 12 Primer for Illumina E7322AAVIAL -20 1 x 0.04 ml 10 µM

Companion Products

• NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®
• NEBNext® Ultra™ II Ligation Module 
• NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
• NEBNext® Ultra Ligation Module
• NEBNext® Quick Ligation Module
• NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
• NEBNext^® Ultra^™ II RNA Library Prep with Sample Purification Beads
• NEBNext^® Ultra^™ II DNA Library Prep with Sample Purification Beads
• NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina
• NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads

1. Refer to the manual (NEB #E7335) for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.

• E7335_SingleIndex_IlluminaLRM
• Sample Sheet Index Sequences E7335

• NEBNext® Multiplex Oligos Selection Chart

1. Are NEBNext adaptors and primers for Illumina validated in Next Generation Sequencing Workflows?
2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
3. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
5. Are these reagents available in a customized format, larger format or 96 well formats?
6. Can USER Enzyme be purchased separately?
7. Are the NEBNext Oligos for Illumina compatible with single read and paired end sequencing?
8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
9. How do I know the concentration of my index primers? 
10. Do you have a Sample Sheet with the index sequences that can be used in the Illumina Experiment Manager (IEM)?
11. How do I use the NEB provided Sample Sheet for my specific instrument?
12. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7335S_L_v1
• E7335G_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7335L_v1_10023878
• E7335L_v1_10030621
• E7335L_v1_10048673
• E7335S_v1_10058949
• E7335L_v1_10065012
• E7335S_v1_10072622
• E7335L_v1_10052909
• E7335L_v1_10098047
• E7335S_v1_10107836
• E7335L_v1_10128611
• E7335L_v1_10133179
• E7335L_v1_10160966
• E7335S_v1_10169924
• E7335S_v1_10245007
• E7335S_v1_10277160
• E7335L_v1_10277391
• E7335S_v1_10287197
• E7335G_v1_10286709

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.