Thermolabile Proteinase K
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
P8111S |
Thermolabile Proteinase K |
30 units ( 120 units/ml ) | - | Unavailable in your region |
Thermolabile Proteinase K
This product is available in a glycerol-free format. Contact us for more information.
Product Introduction
Thermolabile Proteinase K is a recombinant Proteinase K engineered for rapid and complete heat inactivation with broad protease activity, for protein and enzyme degradations in nucleic acid preparations and other applications.
- Recombinant Proteinase K, engineered to be completely heat inactivated at 55°C for just 10 minutes, while maintaining similar activity and specificity as the wild-type version.
- No detectable endonuclease, exonuclease, DNase or RNase contaminating activities. Suitable for sequencing and amplification sample preparation as well as broad molecular biology applications.
- Enables multi-step workflows in the same reaction vessel without the need for bead-based purification.
- Robust activity across a wide range of reaction buffer types, salt concentrations, detergents, as well as DTT and EDTA, with optimal activity between 20 - 40°C and pH 7.0 - 9.5.
Catalog # | Size | Concentration |
---|---|---|
P8111S | 30 units | 120 units/ml |
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Product Information
Description
Thermolabile Proteinase K is a recombinantly engineered, subtilisin-related serine protease that will hydrolyze a variety of peptide bonds. Unlike other Proteinase K versions that can only be partially inactivated by heat, Thermolabile Proteinase K can be entirely heat-inactivated, allowing for simpler, more efficient workflows, without the need for additional purification steps to remove the enzyme. Thermolabile Proteinase K maintains similar activity and specificity to the wild type version, cleaving peptide bonds at the carboxyl side of most amino acid residues, with some preference at aliphatic or aromatic ones.
Thermolabile Proteinase K (TLPK) can be completely inactivated by incubation at 55°C for 10 minutes, which allows for subsequent enzymatic steps in the same reaction vessel.



Using Thermolabile Proteinase K instead of magnetic beads for cleanup in DNA or RNA library prep keeps the reaction in a single tube, streamlining workflows, improving yield, and reducing costs. This eliminates multiple wash steps, minimizes sample loss, and prevents enzyme carryover between steps. It's especially beneficial for low-input samples, automation, and high-throughput applications.
Product Source
Cloned from Engyodontium album (formerly Tritirachium album), mutagenized to increase thermolability of the enzyme and expressed in Pichia pastoris.- This product is related to the following categories:
- Proteases,
- Total RNA Extraction & Purification,
- PCR, qPCR & Amplification Technologies,
- Nucleic Acid Purification,
- This product can be used in the following applications:
- PCR & Reaction Cleanup,
- Protein Analysis Tools,
- Protein Digestion, DNA Amplification, PCR & qPCR
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to release 1.0 µmol of 4-nitroaniline per minute from N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide at 25°C, in a total reaction volume of 105 µl.Storage Buffer
20 mM Tris-HCl
1 mM CaCl2
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
55°C for 10 minutesMolecular Weight
Apparent: 29 kDaUnit Assay Conditions
A series of dilutions of Thermolabile Proteinase K are incubated with 0.25 mM N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide in 0.1% SDS, 0.1% Triton X-100, 20 mM Tris-HCl, 5 mM CaCl2, 50 mM NaCl (pH 8.0 @ 25°C) in a 105 μl reaction. The reaction mix is incubated at 25°C. Liberation of p-nitroaniline is detected by real-time UV spectroscopy at 405 nm.Features
- Break down and inactivate proteins and enzymes with this unique Proteinase K that can be completely and rapidly heat inactivated. Effectively degrades all proteins, requires minimal enzyme for fast reactions, and functions across diverse conditions and workflows
- Enables successive enzymatic reactions in a single reaction vessel without bead purification steps
- Suitable for DNA, RNA extraction, nucleic acid sample preparation for sequencing and amplification applications
- Also available in glycerol-free format
Related Products
Companion Products
Product Notes
- Active in a wide range of buffers. It is highly active between pH 7.0 and 9.5 and temperatures 20-40°C. It is active in chelating agents such as EDTA up to 10 mM.
- Thermolabile Proteinase K is stable for at least 2 years at –20°C. No loss of activity is observed after 10 freeze-thaw cycles.
- Thermolabile Proteinase K is highly active over a broad pH range (7.0 - 9.5) and a wide temperature range (20 – 40°C).
- Thermolabile Proteinase K is active in chelating agents such as EDTA up to 10 mM.
- Enzyme activity is stimulated in up to 1% SDS and no inhibition is observed in SDS concentrations up to 2.5% or Triton X-100 concentrations up to 1.5%.
- Thermolabile Proteinase K activity is inhibited by urea concentrations greater than 2 M.
Protocols, Manuals & Usage
Protocols
Tools & Resources
Selection Charts
FAQs & Troubleshooting
FAQs
- How is Thermolabile Proteinase K different from Proteinase K, Molecular Biology Grade?
- Can I use Thermolabile Proteinase K for molecular biology applications?
- Should I switch from Proteinase K, Molecular Biology Grade to Thermolabile Proteinase K?
- If I switch from Proteinase K, Molecular Biology Grade to Thermolabile Proteinase K, should I use the same amount of enzyme?
- How do I heat inactivate Thermolabile Proteinase K?
- What buffer should I use to dilute Thermolabile Proteinase K?
- What is the optimal reaction buffer for Thermolabile Proteinase K?
- What is the optimal incubation temperature and time?
- Is Thermolabile Proteinase K compatible with EDTA, Triton X-100, SDS, DTT and/or Urea?
- Is Thermolabile Proteinase K active in common NEB buffers?
- Is Thermolabile Proteinase K active in the presence of metal ions?
- Why is the unit definition assay for Thermolabile Proteinase K (NEB #P8111) different from the unit definition assay for Proteinase K, Molecular Biology Grade (NEB # P8107)?
- What is the activity if Proteinase K, Molecular Biology Grade if subjected to the unit definition assay used for Thermolabile Proteinase K?
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- P8111S_v1_10040707
- P8111S_v1_10055881
- P8111S_v1_10058664
- P8111S_v1_10067120
- P8111S_v1_10078222
- P8111S_v1_10085286
- P8111S_v1_10126953
- P8111S_v1_10144324
- P8111S_v1_10155106
- P8111S_v1_10179459
- P8111S_v1_10197130
- P8111S_v1_10212235
- P8111S_v1_10217658
- P8111S_v1_10235620
- P8111S_v1_10244576
- P8111S_v1_10256767
- P8111S_v1_10268510
- P8111L_v1_10281875
- P8111S_v1_10281428
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Thermolabile Proteinase K
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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