Product Class: Other

DNase I (RNase-free)
NEBU cloned at NEB recombinant 37 75 Heat

GMP-grade reagent also available. Learn more.

Product Introduction

  • DNA specific Endonuclease
  • Degrades double-stranded and single-stranded DNA
  • Products are short oligos with 5'-phosphate and 3'-OH
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

DNase I (RNase-free) is ideal for:

  • Removal of contaminating genomic DNA from RNA samples
  • Degradation of DNA templates in transcription reactions
  • Salt-tolerant DNase I-XT (NEB #M0570) is now available for removal of DNA from RNA preparations
Catalog # Size Concentration
M0303S 1000.0 units 2000 units/ml
M0303L 5000.0 units 2000 units/ml

Product Information

Description

Highlights

Isolated from a recombinant source
Supplied with 10X Reaction Buffer DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Product Source

An E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.
This product is related to the following categories:
Exonucleases and Non-specific Endonucleases Products,
RNA Synthesis In vitro Transcription (IVT),
This product can be used in the following applications:
RNA Cloning

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.

Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

Reaction Conditions

1X DNase I Reaction Buffer
Incubate at 37°C

1X DNase I Reaction Buffer
10 mM Tris-HCl
2.5 mM MgCl2
0.5 mM CaCl2
(pH 7.6 @ 25°C)

Usage Concentration

2,000 units/ml

Storage Buffer

10 mM Tris-HCl
2 mM CaCl2
50% Glycerol
pH 7.6 @ 25°C

Heat Inactivation

75°C for 10 minutes

Application Features

  • Degradation of DNA template in transcription reactions
  • Removal of contaminating genomic DNA from RNA samples
  • DNase I footprinting
  • Nick Translation

Product Notes

  1. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3).
  2. We do not recommend using NEB's DNase I as a substitute for Monarch DNase I in the RNA isolation workflow when using the Monarch Total RNA Miniprep Kit (NEB #T2010).  Monarch DNase I is optimized for use in this workflow, and is available for purchase as a component of the Monarch Total RNA Enzyme Pack, (NEB #T2019).

References

  1. Kunitz, M. (1950). J. Gen. Physiol . 33, 349-362.
  2. Vanecko, S. and laskowski, M. (1961). J. Biol. Chem . 236, 3312-3316.
  3. Huang, Z. et al. (1996). Biotechniques . 20, 1012-1020.

Protocols, Manuals & Usage

Protocols

  1. A Typical DNase I Reaction Protocol (M0303)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the specific activity of DNase I(RNase-free)?
  2. Will DNase I work in NEB buffers 1-4?
  3. What is the best way to remove DNase I from my reaction?
  4. Will DNase I work in rCutSmart buffer?
  5. Can I use the Monarch Spin RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
  6. Can I use NEB DNase I (#M0303) with the Monarch Total RNA Miniprep Kit?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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