Product Class: Other

Monarch® Spin Columns S2C and Tubes

Effective August 26, 2024, component/product name changed to Monarch® Spin Columns S2C and Tubes. These columns are now tinted orange.

Product Introduction

The Monarch Spin Columns S2C and Tubes are a component of the Monarch Spin gDNA Extraction Kit (NEB #T3010) and can be used to purify up to 30 µg genomic DNA from a variety of sample types. They are available for purchase separately for your convenience and supplied with Monarch Spin Collection Tubes (NEB #T2118).

Learn more about Monarch DNA and RNA purification products

Catalog # Size Concentration
T3017L 100.0 preps

Product Information

Description

The Monarch Spin Columns S2C and Tubes are a component of the Monarch Spin gDNA Extraction Kit (NEB #T3010) and can be used to purify up to 30 µg of DNA from a wide variety of biological samples. The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. DNA can be eluted in as little as 35 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS, and transfection. Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. They are supplied in quantities of 100, along with 200 Monarch Spin Collection Tubes (NEB #T2118).
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. What is the maximum binding capacity of the Monarch® Spin gDNA Extraction Kit columns?
  2. What is the minimum elution volume that can be used with the Monarch® Spin gDNA Extraction Kit columns?
  3. How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
  4. Can I get better recovery with the Monarch® Spin gDNA Extraction Kit if I do a second elution with my eluent from the first elution?
  5. Can I use a different elution buffer than the one supplied with the Monarch® Spin gDNA Extraction Kit (NEB #T3010)?
  6. What size gDNA can be purified with the Monarch® Spin gDNA Extraction Kit?
  7. What is the minimal input amount that can be used with the Monarch® Spin gDNA Extraction Kit?
  8. Can I use the Monarch® Spin gDNA Extraction Kit to clean up gDNA that was extracted with phenol/chloroform?
  9. Can I use the Monarch® Spin gDNA Extraction Kit for gDNA cleanup?
  10. How can I assess gDNA integrity and purity?
  11. Can I omit the low-speed binding centrifugation step to save time?
  12. Can I omit the RNase A digestion step from the protocol? 
  13. Is Monarch-purified gDNA suitable for long-range PCR?
  14. When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
  15. Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
  16. Why did the silica membrane on my Monarch Spin Columns S2C (NEB #T3017) dislodge during my genomic DNA prep?
  17. Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used to isolate gDNA from drosophila and other insects?
  18. Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used to isolate gDNA from plants?
  19. Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used to isolate gDNA from plasma, serum and urine?
  20. Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used for isolating cfDNA?
  21. Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used with a vacuum manifold?
  22. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?
  23. I noticed that in the Monarch® Spin gDNA Extraction Kit (NEB #T3010), the Monarch Spin Columns S2C (NEB #T3017) for gDNA purification are now tinted orange. Does this impact performance?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective August 26, 2024, component/product name changed to Monarch® Spin Columns S2C and Tubes. These columns are now tinted orange.

Effective May 9, 2022: Shelf life is 24 months.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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