Product Class: Other

Monarch® gDNA Cell Lysis Buffer

Product Introduction

The Monarch gDNA Cell Lysis Buffer is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA from a variety of sample types. This lysis buffer provides mild lysis conditions that help reduce the viscosity that is common in cell samples. The Monarch gDNA Cell Lysis Buffer is one of three lysis buffers available for purchase separately for additional convenience and to minimize waste.
Catalog # Size Concentration
T3012L 20.0 ml

Product Information

Description

The Monarch gDNA Cell Lysis Buffer is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010). It provides mild lysis conditions that help to reduce the viscosity common in cell samples. Optimized for speed and efficiency, this buffer requires only a 5 minute lysis incubation time with Monarch Proteinase K and Monarch RNase A to obtain maximal yields of highly pure and intact gDNA with a typical peak size >60 kb. 
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Quick Protocol for Extraction and Purification of Genomic DNA
  2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
  3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
  4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
  5. Protocol for Genomic DNA Cleanup (NEB #T3010)
  6. Genomic DNA Extraction from Gram-negative Bacteria (NEB #T3010)
  7. Genomic DNA Extraction from Yeast (NEB #T3010)
  8. Genomic DNA Extraction from Saliva (NEB #T3010)
  9. Genomic DNA Extraction from Buccal Swabs (NEB #T3010)
  10. Genomic DNA Extraction from Gram-positive Bacteria and Archaea (NEB #T3010)

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. What is the composition of each buffer provided with the Monarch® Spin gDNA Extraction Kit?
  2. What size gDNA can be purified with the Monarch® Spin gDNA Extraction Kit?
  3. Can I omit the RNase A digestion step from the protocol? 

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective May 9, 2022: Shelf life is 24 months.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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