Product Class: Other

TriDye™ 100 bp DNA Ladder

Product Introduction

  • Size range: 100 bp to 1,517 bp
  • Convenient, ready-to-load format
  • Formulated with 3 different tracking dyes
  • Easy to identify reference bands
  • Suitable for 125 gel lanes

 

Catalog # Size Concentration
N3271S 125.0 gel lanes (1.25 ml) 50 µg/ml

Product Information

Description

TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.

  • Recommended gel percentage range: 1.2-3%
  • Optimum separation on 2%
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.

N3271fig2_thumb
100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment Mass (ng) bp
1 45 1,517
2 35 1,200
3 95 1,000
4 27 900
5 24 800
6 21 700
7 18 600
8 97 500/517
9 38 400
10 29 300
11 25 200
12 48 100

Effective Size Range

100bp to 1,517bp

Storage Buffer

0.006% Xylene Cyanol
10 mM Tris-HCl
10 mM EDTA
10% Glycerol
0.006% bromophenol blue
0.06% Orange G
pH 8 @ 25°C

Product Notes

  1. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  2. We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
  3. In general, it is recommended to load 1ul per mm of gel lane.
  4. TriDye DNA Ladder is stable for at least 6 months at 25°C
  5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

Protocols, Manuals & Usage

Protocols

  1. Protocol for TriDye 100 bp DNA Ladder (NEB #N3271)

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Why are there extra bands visible on polyacrylamide gels?
  4. What are the differences in the four versions of the 1 kb Plus (formerly called the 2-Log DNA Ladder), 100 bp and 1 kb DNA Ladders that NEB sells?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  6. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  7. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Tech Tips

Mix well before use, especially after thawing.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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