Product Class: Other

λ DNA-Mono Cut Mix

Product Introduction

  • The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI.
  • The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing.
  • The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
  • For pulsed-field gel electrophoresis (PFGE)
  • May also be used for standard electrophoresis
  • Size range: 1,503 bp to 48,502 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Catalog # Size Concentration
N3019S 100.0 gel lanes (0.1 ml) 500 µg/ml

Product Information

Description

  • Recommended gel and migration conditions: PFGE on CHEF system, 1% 0.5X TBE agarose, 6V/cm, 15C, 0.5 to 1.5 seconds for 20 hours.


N3019_thumb
PFGE separation of 0.5 µg of Lambda Mono Cut Mix. 1% agarose gel, 0.5X TBE, 6 V/cm, 15°C for 20 hours. Switch times ramped from 0.5-1.5 seconds.
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment Mass (ng) bp
1 100 48502
2 79 38416
3 69 33498
4 62 29946
5 51 24508
6 49 23994
7 35 17053
8 31 15004
9 21 10086
10 3 1503

Effective Size Range

1,503bp to 48,502bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Product Notes

  1. These fragments are best separated by Pulsed Field Gel Electrophoresis. Alternatively Lambda Mono Cut Mix can be separated by conventional gel electrophoresis using the following conditions: 0.5 μg Lambda Mono Cut Mix, 0.4 % agarose gel, 1X TAE, 20-30 V, 25°C for 24 hours.
  2. 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Daniels, D.L. et al. (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.

Protocols, Manuals & Usage

Protocols

  1. Suggested Loading Protocol for DNA Ladders & Markers

     

FAQs & Troubleshooting

FAQs

  1. How can I quantify the amount of DNA in each band of a marker?
  2. General information on Lambda DNA Mono Cut Mix:
  3. Can I use Midori Green with the DNA Ladders from NEB?
  4. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  5. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
  6. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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