λ DNA-BstEII Digest
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
N3014S |
Lambda/BstE II Digest |
150 gel lanes ( 500 µg/ml ) | - | Unavailable in your region |
λ DNA-BstEII Digest
Product Introduction
- Size range: 117 bp to 8,454 bp
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Catalog # | Size | Concentration |
---|---|---|
N3014S | 150.0 gel lanes (0.3 ml) | 500 µg/ml |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
The BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
- Recommended gel percentage range: 0.6-1.2%
- Optimum separation on 0.8%
- This product is related to the following categories:
- DNA Markers & Ladders Products
- This product can be used in the following applications:
- DNA Analysis
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Properties & Usage
Bases
Fragment | Mass (ng) | bp |
---|---|---|
1 | 174 | 8,454 |
2 | 149 | 7,242 |
3 | 131 | 6,369 |
4 | 117 | 5,686 |
5 | 99 | 4,822 |
6 | 89 | 4,324 |
7 | 76 | 3,675 |
8 | 48 | 2,323 |
9 | 40 | 1,929 |
10 | 28 | 1,371 |
11 | 26 | 1,264 |
12 | 14 | 702 |
13 | 5 | 224 |
14 | 2 | 117 |
Effective Size Range
117bp to 8,454bpStorage Buffer
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C
Related Products
Companion Products
- 1 kb DNA Ladder
- 100 bp DNA Ladder
- Gel Loading Dye, Purple (6X), no SDS
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
Materials Sold Separately
Product Notes
- For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
- The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
- 1X Gel Loading Dye, Purple, no SDS:
2.5% Ficoll®-400
10mM EDTA
3.3mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2
References
- Daniels, D.L. et al (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
- Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.
Protocols, Manuals & Usage
Protocols
FAQs & Troubleshooting
FAQs
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- How can I quantify the amount of DNA in each band of a marker?
- Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
- Can I use Midori Green with the DNA Ladders from NEB?
- Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
- Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
Tech Tips
Heat at 60C for 3 minutes to separate the cohesive ends of fragments 1 and 4.
To make it ready-to-load, dilute in TE buffer instead of water.
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- N3014S_L_v1_0831401
- N3014S_L_v1_0831407
- N3014S_L_v1_0821412
- N3014S_L_v1_0821505
- N3014S_L_v1_0831505
- N3014S_L_v1_0821511
- N3014S_L_v1_0831511
- N3014S_L_v1_0821604
- N3014S_L_v1_0831604
- N3014S_L_v1_0821608
- N3014S_L_v1_0831608
- N3014S_L_v1_0821612
- N3014S_L_v1_0831612
- N3014S_L_v1_0821704
- N3014S_L_v1_0831704
- N3014S_L_v1_0831706
- N3014S_L_v1_0831711
- N3014S_L_v1_0831802
- N3014L_v1_10011631
- N3014S_v1_10008859
- N3014S_v1_10038756
- N3014S_v1_10046697
- N3014S_v1_10060478
- N3014S_v1_10070363
- N3014S_v1_10098962
- N3014S_v1_10117622
- N3014S_v1_10141661
- N3014S_v1_10168637
- N3014S_v1_10194196
- N3014S_v1_10236649
- N3014S_v1_10263049
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.λ DNA-BstEII Digest
Gel Loading Dye, Purple (6X), no SDS
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.