Product Class: Other

XRN-1
NEB3 recombinant 37

New size

Product Introduction

XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. It also acts on 5´ monophosphate ssDNA with greatly reduced efficiency.

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of DNases and RNases
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.
Catalog # Size Concentration
M0338S 20.0 units 1000 units/ml
M0338L 100.0 units 1000 units/ml

Product Information

Description

XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. It also acts on 5´ monophosphate ssDNA with greatly reduced efficiency.

Product Source

Purified from E. coli carrying a plasmid overexpressing the yeast XRN-1 gene (1).
This product is related to the following categories:
RNases,
RNA Modification,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that digests 1 µg of phosphorylated yeast RNA in 60 minutes at 37°C.

Reaction Conditions

1X NEBuffer™ 3
Incubate at 37°C

1X NEBuffer™ 3
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

20 mM Tris-HCl
500 mM NaCl
2 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100

Product Notes

  1. We recommend adding Murine RNase Inhibitor (NEB #M0314) in your reaction at 1 unit/µl to prevent non-specific RNA degradation.

References

  1. Stevens, A (1980). Biol. Chem. 255, 3080-3085.

Protocols, Manuals & Usage

Protocols

  1. One-pot digestion of 5´ triphosphorylated RNA using RppH (NEB #M0356) & XRN-1 (NEB #M0338)
  2. Protocol for XRN-1 NEB M0338

FAQs & Troubleshooting

FAQs

  1. What is the molecular weight of XRN-1?
  2. Is XRN-1 a tagged protein?
  3. Can XRN-1 digest ssDNA containing 5’ monophosphates?
  4. Is XRN-1 identified with other names in the literature?

Tech Tips

We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In