Product Class: Other

RNase Inhibitor, Human Placenta
recombinant

Product Introduction

RNase Inhibitor, Human Placenta, specifically inhibits RNases A, B and C.

  • Isolated from a recombinant source
  • Inhibits common eukaryotic RNases
  • Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases
  • Active over a broad pH range (pH 5-8)
  • Tested for the absence of DNases and RNases
Catalog # Size Concentration
M0307S 2000.0 units 40000 units/ml
M0307L 10000.0 units 40000 units/ml

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Product Information

Description

RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014 (2).

Product Source

An E. coli strain that carries the Ribonuclease Inhibitor gene from human placenta.
This product is related to the following categories:
RNase Inhibitors,
RNA Synthesis In vitro Transcription (IVT),
This product can be used in the following applications:
RNA Cloning,
RT-PCR & cDNA Synthesis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of RNase Inhibitor, Human Placenta required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2, 3'-cyclic monophosphate by RNase A.

Storage Buffer

50 mM KCl
8 mM DTT
50% Glycerol
20 mM HEPES-KOH
pH 7.6 @ 25°C

Product Notes

  1. Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.
  2. The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are possible sources of RNase contamination (i.e. enzymes, plasmid from a mini prep.)

References

  1. Blackburn, P. and Moore, S. (1982). Pancreatic Ribonucleases . The Enzymes. XV, Part B, Academic Press, NY.
  2. Blackburn, P., Wilson, G. and Moore, S. (1977). J. Biol. Chem. 252,5904.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Avoiding Rnase Contamination using Rnase Inhibitor, Human Placenta (M0307)

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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