Product Class: Other

Hemo KlenTaq®
NEBU recombinant No extraction-free PCR pcr 5

Product Introduction

Eliminate DNA extraction and purification

  • Perform PCR directly from whole blood samples
  • Bypass costly DNA extraction and purification steps
  • Amplify from limited quantities of DNA
Catalog # Size Concentration
M0332S 200.0 reactions
M0332L 1000.0 reactions

Product Information

Description

Hemo KlenTaq is a truncated version of Taq DNA Polymerase, lacking the first 280 amino acids (1). Hemo KlenTaq also contains mutations that make it resistant to inhibitors present in whole blood. It can amplify directly from whole blood from humans and mice. Hemo KlenTaq tolerates up to 20% whole blood in a 25 µl reaction (30% in 50 µl reaction).

Amplification of human whole blood with Hemo KlenTaq. Percent blood present in sample and anticoagulant used are indicated in the legend. Ladder M is the 2-Log DNA Ladder (NEB #N3200).

Product Source

An E. coli strain that carries a mutant Taq DNA polymerase gene. The protein lacks the N-terminal 5´→ 3´ exonuclease domain and the gene has three internal point mutations.
This product is related to the following categories:
Other PCR Polymerases Products
This product can be used in the following applications:
Extraction-Free PCR,
Specialty PCR,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • M0332S     -20    
      Hemo KlenTaq® M0332SVIAL -20 1 x 0.4 ml 200 reactions
      Hemo KlenTaq® Reaction Buffer Pack B0332SVIAL -20 1 x 1.5 ml 5 X
  • M0332L     -20    
      Hemo KlenTaq® M0332LVIAL -20 2 x 1 ml 500 reactions
      Hemo KlenTaq® Reaction Buffer Pack B0332SVIAL -20 4 x 1.5 ml 5 X

Properties & Usage

Reaction Conditions

1X Hemo KlenTaq® Reaction Buffer Pack

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

5' - 3' Exonuclease

No

3' - 5' Exonuclease

No

Resulting Ends

  • Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

Features

  • Versatility and robustness of Taq DNA Polymerase to amplify DNA from whole blood.

Application Features

  • Whole Blood PCR
  • Dried Blood Spot PCR
  • Primer extension

References

  1. Kermekchiev, M.B., et al. (2009). Nucleic Acids Res. 37, e40.
  2. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques . 15, 372-374.

Protocols, Manuals & Usage

Protocols

  1. PCR Protocol for Hemo KlenTaq®

FAQs & Troubleshooting

FAQs

  1. What ends will my PCR products have?
  2. How should I determine the appropriate annealing temperature for my reaction?
  3. My results are not as expected. Where can I find troubleshooting help?

Tech Tips

For best results, slowly pipette your blood sample into the reaction last and let it settle to the bottom of the reaction. The reaction should not be mixed once the blood is added.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

NEW ENGLAND BIOLABS® and THERMOPOL® is a registered trademark of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
KLENTAQ® is a registered trademark of DNA Polymerase Technology, Inc.
PRIMERSELECT™ is a trademark of DNAStar, Inc.
TWEEN® is a registered trademark of Uniqema Americas, LLC.
Licensed under U.S. Patent No. 7,462,475.

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