Product Class: Other

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

Product Introduction

This module is part of the Ultra™ II Directional RNA workflow, for Illumina®- compatible strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 10 ng – 1 µg total RNA.

Kits are also available that include reagents for every step in the Ultra II Directional RNA library construction workflow (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB #E7760 and NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, NEB #E7765).
Catalog # Size Concentration
E7550S 24.0 reactions
E7550L 96.0 reactions

Product Information

Description




The NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library preparation workflow.  This workflow uses the “dUTP” method for strand-specific library construction, which depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix, included in this module.

The NEBNext Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:

This product is related to the following categories:
Automation for NEBNext® NGS Library Prep Products,
RNA Library Prep for Illumina,
Next Generation Sequencing Library Preparation Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E7550S     -20    
      NEBNext Second Strand Synthesis Enzyme Mix E7425AVIAL -20 1 x 0.096 ml Not Applicable
      NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix E7426AVIAL -20 1 x 0.192 ml Not Applicable
  • E7550L     -20    
      NEBNext Second Strand Synthesis Enzyme Mix E7425AAVIAL -20 1 x 0.384 ml Not Applicable
      NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix E7426AAVIAL -20 1 x 0.768 ml Not Applicable

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Protocols, Manuals & Usage

Protocols

  1. Protocol for NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. What is the difference between the NEBNext Ultra II Directional RNA Second Strand Module and the NEBNext mRNA Second Strand Synthesis Module?
  2. For the downstream workflow, which Modules for End Repair, dA-Tailing and Ligation should be used with the NEBNext Ultra Directional Second Strand Synthesis Module?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Limited Use Label License:  This product and its use are subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft.  The purchase of this product from New England Biolabs, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for profit entity) or for limited commercial purposes that are pre-approved by New England Biolabs, Inc.  The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications directed to producing the product.  The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use this product for the following COMMERCIAL PURPOSES:  (1) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in humans or animals.

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