Product Class: Other

NEBNext® Single Cell Lysis Module

Product Introduction

This module contains the NEBNext® Cell Lysis Buffer and Murine RNase Inhibitor, which are optimized for lysis and storage of cells for use in the NEBNext Single Cell/Low Input RNA Library Prep workflow.
 
This module can be used to supplement the NEBNext® Cell Lysis Buffer and Murine RNase Inhibitor components supplied in the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB #E6420) and the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB #E6421).
 
Catalog # Size Concentration
E5530S 96.0 reactions

Product Information

Description

This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420) or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (NEB #E6421), and can be used to supplement the NEBNext® Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.

This product is related to the following categories:
RNA Library Prep for Illumina,
Next Generation Sequencing Library Preparation Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E5530S     -20    
      Murine RNase Inhibitor E6429AAVIAL -20 1 x 0.096 ml Not Applicable
      NEBNext® Cell Lysis Buffer E6428AAVIAL -20 1 x 0.192 ml Not Applicable

Properties & Usage

Materials Required but not Supplied

  • Nuclease-free Water
  • DNA LoBind Tubes (Eppendorf® #022431021) 
  • Thermocycler 
  • Microcentrifuge

Protocols, Manuals & Usage

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. What cell types does the NEBNext Single Cell Lysis Module (NEB #E5530) support?
  2. Can I resuspend the cells in buffers/medium other than PBS before cell collection?
  3. Do the cells have to be sorted in to a specific reagent?
  4. Can frozen cells be used?
  5. Where can I find protocols for low-input RNA library prep for use with the following kits: NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420), NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB #E6421), and NEBNext® Single Cell Lysis Module (#E5530)?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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