Product Class: Kit

Taq PCR Kit

Product Introduction

The industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Kit format includes optimized reagents and enables flexible experimental design for 200 PCR reactions
Catalog # Size Concentration
E5000S 200.0 reactions

Product Information

Description

Highlights

Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides

The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad-range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.

This product is related to the following categories:
Taq DNA Polymerase Products,
This product can be used in the following applications:
Multiplex PCR,
Specialty PCR,
Routine PCR,
PCR

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

References

  1. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
  2. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  3. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  4. Lawyer, F.C. et al. (1993). PCRMethod and Appl.. 2, 275-287.
  5. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
  6. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  7. Powell, L.M. et al. (1987). Cell. 50, 831-840.

Protocols, Manuals & Usage

Protocols

  1. Protocol for a Routine Taq PCR
  2. Taq DNA Polymerase Guidelines for PCR Optimization

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What ends will my PCR products have?
  2. How should I determine the appropriate annealing temperature for my reaction?
  3. What should the final primer concentration be in my PCR?
  4. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  5. What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
  6. Is this Taq DNA Polymerase product compatible with other NEB Buffers?
  7. Can Taq/OneTaq® DNA Polymerases be used for nick translation?
  8. How can I optimize my PCR when using Taq DNA Polymerase?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

ONETAQ®, QUICK-LOAD®, NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas LLC.

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