Product Class: Other

ThermoPol® Reaction Buffer

Product Introduction

Obtain high product yields under demanding conditions

  • Recommended and compatible with popular thermophilic DNA polymerases
  • Supplemental MgSO4 included for reaction optimization
Catalog # Size Concentration
B9004S 6.0 ml 10 X

Product Information

Description

10X ThermoPol Reaction Buffer is optimized for use with Vent® and Deep Vent® DNA Polymerases. This buffer also provides superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes. Supplemental MgSO4 is provided to maximize flexibility for users that require additional Mg2+ for their specific application. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.
This product is related to the following categories:
Reaction Buffers Products,
Taq DNA Polymerase Products,
Buffers Products,
This product can be used in the following applications:
Colony PCR,
A-tailing,
Isothermal Amplification,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

1X Buffer Components

20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
pH 8.8@25°C

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is this Taq DNA Polymerase product compatible with other NEB Buffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS®, VENT® and THERMOPOL® are registered trademarks of New England Biolabs, Inc. 
DEEP VENT® is a trademark of New England Biolabs, Inc. 

TRITON® is a registered trademark of Union Carbide Corporation.

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