LunaScript® Multiplex One-Step RT-PCR Kit
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
E1555S |
LunaScript Multiplex One-Step RT-PCR Kit |
50 rxns | - | Unavailable in your region | |
E1555L |
LunaScript Multiplex One-Step RT-PCR Kit |
250 rxns | - | Unavailable in your region |
LunaScript® Multiplex One-Step RT-PCR Kit
Catalog #E1555
Product Introduction
- Features thermostable Luna® WarmStart® Reverse Transcriptase and Q5® Hot Start High-Fidelity DNA Polymerase
- Detect as low as 0.01 pg of human total RNA
- Multiplexing capacity supports use in ARTIC workflows
- Aptamer-based enzyme control enables room temperature setup and stability up to 24 hours
- Save time, plastics, and minimize contamination with a closed-tube, one-step RT-PCR protocol
- Learn how NEB is supporting COVID-19 research with ARTIC-based virus sequencing solutions and RT-qPCR and RT-LAMP virus detection options
- Looking for an RT-qPCR mix? Consider the Luna® Probe One-Step RT-qPCR 4X Mix with UDG or view other qPCR product options
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Intended for RT-PCR, the LunaScript Multiplex One-Step RT-PCR Kit offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. The 5X reaction mix contains dNTPs and is optimized for multiple target detection in a simple workflow. The 25X enzyme mix features Luna WarmStart Reverse Transcriptase and Q5 Hot Start High-Fidelity DNA Polymerase. The dual-temperature control of enzyme activities by aptamer-based inhibition enables room temperature reaction setup, with preassembled reactions stable at room temperature for up to 24 hours. The kit has robust multiplex target amplification capacity and enables various applications such as diagnostics, pathogen detection, and viral genome sequencing (including the ~50 amplicons per reaction used in ARTIC SARS-CoV-2 sequencing protocols).


Libraries were constructed from 1.3 μl of each sample of pooled amplicons using the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) and sequenced on a MiSeq® instrument (2X75 bp). Sequencing reads per amplicon were plotted for each library. Reads were downsampled with seqtk (250,000 read pairs) and aligned to the SARS-CoV-2 reference genome (NCBI, NC_045512) with Bowtie 2. Reference line indicates typical quality threshold of 100 reads.


- This product is related to the following categories:
- RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases Products,
- This product can be used in the following applications:
- RT-PCR,
- cDNA Synthesis,
- RT-PCR & cDNA Synthesis
Properties & Usage
Features
- Streamlined one-step RT-PCR protocol with Luna WarmStart Reverse Transcriptase and Q5 Hot Start High-Fidelity DNA Polymerase
- High sensitivity: detect as low as 0.01 pg of human total RNA
- Superior multiplexing capacity
- Up to 24 hours of room temperature stability
Related Products
Companion Products
- LunaScript™ RT SuperMix Kit
- LunaScript® RT Master Mix Kit (Primer-free)
- NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina
- NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
- NEBNext® Ultra™ II End Repair/dA-Tailing Module
- NEBNext® Ultra™ II Ligation Module
- NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)
Protocols, Manuals & Usage
Protocols
Manuals
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- Can I set up my LunaScript Multiplex One-Step RT-PCR reactions at room temperature?
- Can I add random hexamers or oligo (dT) primers to LunaScript Multiplex One-Step RT-PCR reactions?
- Can I reduce the reaction volume in the LunaScript Multiplex One-Step RT-PCR reactions?
- How can I determine the annealing temperature for my LunaScript Multiplex One-Step RT-PCR reactions?
- Can I increase the reverse transcription time beyond the 10 minutes recommended in the LunaScript Multiplex One-Step RT-PCR protocol?
- Are the PCR products generated with the LunaScript Multiplex One-Step RT-PCR Kit compatible with a TA cloning system?
- What is the lowest RNA input amount recommended for the LunaScript Multiplex One-Step RT-PCR Kit?
- What can I do if a particular amplicon failed in a multiplex reaction using the LunaScript Multiplex One-Step RT-PCR Kit?
- Can I use the LunaScript Multiplex One-Step RT-PCR Kit to amplify long amplicons?
- Can the LunaScript Multiplex One-Step RT-PCR Kit be used in the ARTIC workflow for RNA viral genome sequencing?
- How do I choose between One-Step RT-PCR and Two-Step RT-PCR protocols?
- How do I choose between LunaScript Multiplex One-Step RT-PCR Kit and OneTaq One-Step RT-PCR Kit (NEB #E5315)?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- E1555L_v1_10120295
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Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
Notice to Purchaser: Nucleic acid-based aptamers for use with reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 7,947,447; 8,404,830; 8,975,388 and their foreign equivalents). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart® Luna Reverse Transcriptase for Research Purposes Only. Commercial use of the WarmStart® Luna Reverse Transcriptase requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.