NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
C2992I |
NEB 5-alpha F' lq Competent E. coli (High Efficiency) |
6 x 0.2 ml | - | Unavailable in your region | |
C2992H |
NEB 5-alpha F´ lq Competent E. coli (High Efficiency) |
20 x 0.5 ml | - | Unavailable in your region |
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
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Product Introduction
NEB 5-alpha F'Iq Competent E. coli is a derivative of the popular DH5α.
- Extremely high transformation efficiency suitable for toxic gene cloning
- Tight expression control (lacIq)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Catalog # | Size | Concentration |
---|---|---|
C2992H | 20.0 x 0.05 ml | |
C2992I | 6.0 x 0.2 ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Highlights
- DH5α™ derivative
- Free of animal products
- Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
- Tight control of expression by laclq allows potentially toxic genes to be cloned
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA2)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene
- F' allows cells to be infected with bacteriophage M13 for ssDNA production
- Reduced recombination of cloned DNA (recA1)
- K12 Strain
Transformation Efficiency
1-3 x 109 cfu/µg pUC19 DNA Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.Genotype
F´ proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) / fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17- This product is related to the following categories:
- Cloning Competent Cell Strains Products
- This product can be used in the following applications:
- Transformation,
- High-throughput cloning and automation solutions
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||||||||
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Properties & Usage
Antibiotic for Plasmid Selection
Antibiotics for Plasmid Selection | Working Concentration |
---|---|
Ampicillin | 100 µg/ml |
Carbenicillin | 100 µg/ml |
Chloramphenicol | 33 µg/ml |
Kanamycin | 30 µg/ml |
Streptomycin | 25 µg/ml |
Shipping Notes
- Ships on dry ice
Antibiotic Resistance
- tetracycline
Features
- Toxic gene cloning
- F´ strain with extremely high transformation efficiency
Application Features
Related Products
Companion Products
- NEB® 5-alpha Competent E. coli (High Efficiency)
- NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
Materials Sold Separately
Product Notes
- CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
- STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
Application Notes
Tools & Resources
Selection Charts
Web Tools
FAQs & Troubleshooting
FAQs
- Which competent cell strains are compatible with Gateway® Cloning?
- Does plasmid size affect transformation efficiency (C2992)?
- How long should I incubate cells on ice after DNA has been added (NEB #C2992H and NEB #C2992I)?
- How should I calculate the transformation efficiency (C2992)?
- What are the solutions/recipes (C2992)?
- What are the strain properties (C2992)?
- What is the difference between NEB #C2992H and NEB #C2992I?
- What is the optimal heat shock time for this strain (NEB #C2992H and NEB #C2992I)?
- Which strain of Competent E.coli should I use for general cloning?
- Can I store competent cells at -20°C instead of -80°C?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- What is the shelf life for this strain (NEB #C2992H and NEB #C2992I)?
- Are NEB's competent cells compatible with the “Mix & Go" protocol?
- How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
- How should fragments be prepared for assembly using NEBuilder HiFi?
Citations & Technical Literature
Citations
Additional Citations
- Firnberg E, Ostermeier M (2013) The genetic code constrains yet facilitates Darwinian evolution Nucleic Acids Res; 41(15), 7420-8. PubMedID: 23754851, DOI: 10.1093/nar/gkt536
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- C2992H_I_v1_1311607
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Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)
SOC Outgrowth Medium
pUC19 Vector
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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The supporting documents available for this product can be downloaded below.