Product Class: Other

NEB® Stable Competent E. coli (High Efficiency)

Supplied with outgrowth medium optimized for NEB 10-beta & NEB Stable Competent E.coli ; please do not use SOC outgrowth medium.

Product Introduction

Chemically competent E. coli cells suitable for high efficiency transformation.

  • Ideal for isolation of plasmid clones containing repeat elements and unstable inserts
  • Useful for isolating and propagating retroviral/lentiviral clones
  • Carries the endA mutation (in contrast to Stbl3) so that isolated plasmids are free of Endonuclease I
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Catalog # Size Concentration
C3040H 20.0 x 0.05 ml
C3040I 6.0 x 0.2 ml

Protocols, Manuals & Usage

Protocols

  1. 5 Minute Transformation Protocol for NEB® Stable Competent E. coli (C3040H/C3040I)
  2. High Efficiency Transformation for NEB® Stable Competent E. coli (C3040H)
  3. Protocol for cloning DNA containing repeat elements (C3040)
  4. High Efficiency Transformation Protocol (C3040I)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Which competent cell strains are compatible with Gateway® Cloning?
  2. What are the strain properties (C3040)?
  3. What is the shelf-life for this strain?
  4. Which strain of Competent E.coli should I use for general cloning?
  5. Are NEB Stable Competent E. coli cells suitable for transformation of large plasmids and large NEBuilder HiFi, Gibson Assembly or Golden Gate Assembly products?
  6. How should I calculate the transformation efficiency (C3040)?
  7. What is the optimal heat shock for this strain? (C3040)
  8. What volume of DNA can be added into competent cells?
  9. Is NEB Stable suitable for larger plasmid transformations?
  10. Is NEB Stable a substitute for Stbl2 or Stbl3?
  11. What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
  12. How should I store the NEB 10-beta/Stable Outgrowth Medium?
  13. Do NEB Stable Competent E.coli require an outgrowth recovery period after transformation with AmpR plasmids?
  14. How should fragments be prepared for assembly using NEBuilder HiFi?