Supplied with outgrowth medium optimized for NEB 10-beta & NEB Stable Competent E.coli ; please do not use SOC outgrowth medium.
Now available in 96-well plate format.
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
High efficiency strain ideal for cloning large plasmids and BACs
Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.
Highlights
DH10B™ derivative
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
Reduced recombination of cloned DNA (recA1)
Clone large plasmids and BACs
Free of animal products
K12 Strain
Transformation Efficiency
For C3019I and C3019H: 1–3 x 109 cfu/µg pUC19 DNA
For C3019P: 1–3 x 108 cfu/µg pUC19 DNA
Effect of plasmid size on transformation efficiency NEB 10-beta chemically competent cells (C3019H) are more efficiently transformed with large plasmids than NEB 5-alpha cells (C2987H). The difference in TE between the two cell lines increases with the size of the plasmid being transformed. Effect of heat shock time on NEB 10-beta competent E. coli transformation efficiency 50 μl of competent cells (C3019H) were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds. Effect of DNA incubation time on NEB 10-beta competent E. coli transformation efficiency 50 μl of competent cells (C3019H) were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes. Effect of outgrowth medium on transformation efficiency 50 μl of NEB 10-beta competent E. coli (C3019H) was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB 10-beta/Stable Outgrowth Medium delivers the highest transformation efficiency. Benefit from the high transformation efficiencies of NEB 10-beta (C3019H/I) Transformation efficiencies were compared using manufacturers’ recommended protocols. Values shown are the average of triplicate experiments.
STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
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Specifications & Change Notifications
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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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Trademarks
ONESHOT® and MAX EFFICIENCY® are registered trademarks of Thermo Fisher Scientific. DH10B™ is a trademarks of Thermo Fisher Scientific.
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50 μl of competent cells (C3019H) were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
50 μl of competent cells (C3019H) were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
