Product Class: Kit

NEB Cloning Competent E. coli Sampler

Product Introduction

Not sure which cloning strain to choose? The NEB Cloning Competent E.coli Sampler allows you to sample 4 of our popular chemically competent strains. The Sampler includes two 0.05 ml tubes of each of the following strains (as well as outgrowth medium and control):

  • NEB 5-alpha: Our best-selling strain for basic cloning; Derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
  • NEB 10-beta: derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high-quality plasmid preparations.
  • NEB Stable: Ideal for isolation of plasmid clones containing repeat elements and unstable inserts. Useful for isolating and propagating retroviral/lentiviral clones
  • NEB Turbo: Features fast colony growth (6.5 hours) and tight expression control (lacIq). Isolate DNA after only 4 hours of growth
Catalog # Size Concentration
C1010S 8.0 x 0.05 ml

Product Information

Description

Highlights

  • Transformation efficiencies of 1-3 x 109 cfu/μg pUC19 DNA
  • Resistance to phage T1 (fhuA2)
  • Outgrowth medium and control plasmid included
  • Free of animal products
  • Value pricing
  • No dry ice charges
A sample pack of four cloning strains of E. coli suitable for high efficiency transformation.
This product is related to the following categories:
Competent Cells Products,
Cloning Competent Cell Strains Products,
This product can be used in the following applications:
Transformation

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Product Notes

  1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.

  2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Why did Synthetic Biologist Chris Voigt of MIT choose NEB 10-beta for DNA assembly and cloning?
  2. How should fragments be prepared for assembly using NEBuilder HiFi?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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