Product Class: Kit

NEBuffer™ Set (EcoRI/SspI, DpnII)

Product Introduction

New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity.

Set contains two 1.25ml vials of each unique NEBuffer: EcoRI/SspI and DpnII.

Catalog # Size Concentration
B7006S 1.25 ml each

Product Information

Description

4 vials of NEBuffers for restriction enzymes requiring unique buffers ((2 x EcoRI/SspI, 2 x DpnII)

New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

Set contains two 1.25ml vials of each unique NEBuffer: EcoRI/SspI and DpnII.

1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
0.025% Triton® X-100
(pH 7.5 @ 25°C)

1X NEBuffer™ DpnII
50 mM Bis-Tris-HCl
100 mM NaCl
10 mM MgCl2
0.1 mM DTT
(pH 6 @ 25°C)

 

This product is related to the following categories:
Restriction Endonuclease Buffers & Diluents Products,
Buffers Products,
This product can be used in the following applications:
Cloning Ligation

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • B7006S     -20    
      NEBuffer™ EcoRI/SspI B0101SVIAL -20 2 x 1.25 ml 10 X
      NEBuffer™ DpnII B0543SVIAL -20 2 x 1.25 ml 10 X

Properties & Usage

FAQs & Troubleshooting

FAQs

  1. Can I use NEBuffer EcoRI/SspI with SspI-HF?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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