DpnII
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0543S |
DpnII, recombinant |
1.000 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0543T |
DpnII, recombinant, conc. |
1.000 units ( 50000 units/ml ) | - | Unavailable in your region | |
R0543L |
DpnII, recombinant |
5.000 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0543M |
DpnII, recombinant, conc. |
5.000 units ( 50000 units/ml ) | - | Unavailable in your region |
DpnII
DpnII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10174818. Learn more. We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
Product Introduction
- Time-Saver™ qualified for digestion in 5-15 minutes
- Restriction Enzyme Cut Site: /GATC
Catalog # | Size | Concentration |
---|---|---|
R0543S | 1000.0 units | 10000 units/ml |
R0543T | 1000.0 units | 50000 units/ml |
R0543L | 5000.0 units | 10000 units/ml |
R0543M | 5000.0 units | 50000 units/ml |
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- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Product Source
An E. coli strain that carries the DpnII gene from Diplococcus pneumoniae G41 (S. Lacks).- This product is related to the following categories:
- Methylation Sensitive Restriction Enzymes for Epigenetics Products,
- Methylation Sensitive and Methylation Dependent Restriction Enzymes for Epigenetics,
- Restriction Enzymes for Epigenetic Analysis,
- Restriction Endonucleases C G Products,
- Epigenetic Analysis Products,
- DNA Methylation Analysis,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- Restriction Enzymes for Epigenetics,
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X NEBuffer™ DpnII
Incubate at 37°C
1X NEBuffer™ DpnII
50 mM Bis-Tris-HCl
100 mM NaCl
10 mM MgCl2
0.1 mM DTT
(pH 6 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 25%NEBuffer™ r2.1: 25%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 25%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutesMethylation Sensitivity
dam methylation: Blocked
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Isoschizomers
Related Products
Companion Products
Product Notes
- DpnII and Sau3AI are isoschizomers of MboI.
- Cleaves to leave a 5´ GATC extension which can be efficiently ligated to DNA fragments generated by BamHI, BclI, BglII, MboI, Sau3AI and BstYI.
- This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.
- Will exhibit star activity in NEBuffer r3.1. We recommend the use of NEB DpnII Unique Buffer.
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction Enzymes for Droplet Digital PCR (ddPCR)
- Single Letter Codes
- Star Activity
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5' Overhangs
- Restriction Enzymes for Epigenetics Selection Chart
- Time-Saver™ Qualified Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?
Troubleshooting
Tech Tips
Citations & Technical Literature
Citations
Additional Citations
- Hughes J.R., Roberts N., McGowan S., Hay D., Giannoulatou E., Lynch M., De Gobbi M., Taylor S., Gibbons R., Higgs D.R. (2014) Analysis of hundreds of cis-regulatory landscapes at high resolution in a single, high-throughput experiment Nat Genet; 46 (2), 205-212. PubMedID: 24413732, DOI: doi:10.1038/ng.2871
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0543S_L_v1_0111402
- R0543S_L_v1_0101211
- R0543S_L_v1_0101304
- R0543S_L_v1_0101308
- R0543S_L_v1_0111407
- R0543T_M_v1_0101211
- R0543T_M_v1_0101304
- R0543T_M_v1_0101308
- R0543T_M_v1_0111402
- R0543T_M_v1_0111407
- R0543T_M_v1_0121501
- R0543S_L_v1_0111412
- R0543S_L_v1_0131504
- R0543T_M_v1_0141506
- R0543S_L_v1_0141506
- R0543T_M_v1_0141510
- R0543S_L_v1_0141511
- R0543S_L_v1_0151604
- R0543T_M_v1_0151604
- R0543S_L_v1_0151608
- R0543T_M_v1_0151608
- R0543T_M_v1_0151702
- R0543S_L_v1_0151707
- R0543S_L_v1_0151801
- R0543T_M_v1_0161803
- R0543L_v1_10014860
- R0543M_v1_10014862
- R0543T_v1_10008607
- R0543S_v1_10021263
- R0543T_v1_10025261
- R0543L_v1_10021746
- R0543M_v1_10025259
- R0543S_v1_10021745
- R0543L_v1_10033528
- R0543S_v1_10034609
- R0543M_v1_10042052
- R0543T_v1_10050133
- R0543L_v1_10051233
- R0543S_v1_10055674
- R0543M_v1_10062609
- R0543T_v1_10063600
- R0543S_v1_10064905
- R0543M_v1_10069115
- R0543L_v1_10068575
- R0543M_v1_10076541
- R0543M_v1_10079964
- R0543M_v1_10087342
- R0543L_v1_10087344
- R0543S_v1_10087345
- R0543T_v1_10095481
- R0543M_v1_10095482
- R0543T_v1_10104548
- R0543S_v1_10104220
- R0543L_v1_10107052
- R0543S_v1_10107055
- R0543M_v1_10117540
- R0543S_v1_10122653
- R0543S_v1_10131715
- R0543T_v1_10131712
- R0543M_v1_10136137
- R0543L_v1_10140675
- R0543T_v1_10150682
- R0543M_v1_10150681
- R0543L_v1_10154650
- R0543S_v1_10154647
- R0543L_v1_10170420
- R0543S_v1_10170422
- R0543S_v1_10175255
- R0543M_v1_10170421
- R0543L_v2_10174991
- R0543M_v2_10174985
- R0543S_v2_10174990
- R0543T_v2_10174986
- R0543M_v2_10178839
- R0543M_v2_10183011
- R0543M_v2_10192197
- R0543M_v2_10200532
- R0543T_v2_10192196
- R0543L_v2_10205265
- R0543M_v2_10203054
- R0543S_v2_10203055
- R0543M_v2_10207491
- R0543L_v2_10230646
- R0543S_v2_10230205
- R0543M_v2_10229624
- R0543S_v2_10231000
- R0543S_v2_10238077
- R0543L_v2_10242816
- R0543M_v2_10250228
- R0543T_v2_10251732
- R0543S_v2_10263810
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.DpnII
NEBuffer™ DpnII
Gel Loading Dye, Purple (6X)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.