PCR clean up and gel extraction in one! The two in one kit from MACHEREY-NAGEL*.

• High recoveries for small fragments down to 50 bp

• Separate buffers for single stranded DNA / RNA or SDS containing samples available

• Purify DNA from up to 10 mL reactions or 10 g agarose gels

 

	NucleoSpin® Gel and PCR Clean-up XS (740611.10 / .50 / .250)	NucleoSpin® Gel and PCR Clean-up (740609.10 / .50 / .250)	NucleoSpin® Gel and PCR Clean-up Midi (740986.20)	NucleoSpin® Gel and PCR Clean-up Maxi (740610.20)
Technology	Silica membrane, XS format	Silica membrane technology	Silica membrane technology	Silica membrane technology
Sample material	< 200 μl PCR reaction mixture < 200 mg agarose gel	< 400 μL PCR reaction mixture < 400 mg TAE / TBE agarose gel	< 4 mL PCR reaction mixture < 4 g TAE / TBE agarose gel	< 10 mL PCR reaction mixture < 10 g TAE / TBE agarose gel
Fragment size	50 bp−approx. 20 kbp	50 bp−approx. 20 kbp	50 bp−approx. 20 kbp	50 bp−approx. 20 kbp
Typical recovery	70−95 %	70−95 %	70−95 %	70−95 %
Elution volume	6−12 μL	15−30 μL	200−400 μL	1000 μL
Binding capacity	5 μg	25 μg	75 μg	250 μg
Preparation time	15 min/6 preps	10 min/6 preps	25 min/6 preps	30 min/6 preps
	Learn more	Learn more	Learn more	Learn more

 

 

Application Data

PCR clean up: High recovery down to 50 bp High recovery rates were achieved for fragments down to 50 bp, demonstrating the ability to reliably purify even short fragments into as little as 15 μL of elution volume (15 μL elution volume in dark blue, 30 μL elution volume in blue, and 50 μL in light blue).	Gel extraction: High recovery for a wide range of fragment sizes A high extraction yield was reliably reached across the fragment size span.

 

Do you search for other Clean up products?

Download the DNA and RNA clean up guide

 

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We have samples availble for testing and we are happy to tell you more about these products.

 

 

 

*BIOKÉ distributes MACHEREY-NAGEL products in the Netherlands only.