Principle

Highly efficient clean-up and concentration of RNA samples

The NucleoSpin® RNA Clean-up XS kit is designed for clean-up and concentration of pre-purified RNA samples. Typical sample material covers picogramm to microgramm amounts of pre-purified RNA (e.g., phenol-purified RNA) and RNA from reaction mixtures (e.g., DNase treated samples). The innovative column design with a funnel shaped thrust ring and a small silica membrane area allows processing of sample volumes of up to 300 µL and elution of RNA in as little as 5–30 µL. Thus, highly concentrated RNA is eluted, ready-to-use for sensitive downstream applications (e.g., RT-PCR). RNA enrichment of 20x up to 50x can be achieved. The RNA recovery is typically about 85–95%. High quality RNA (RNA Integrity Number (RIN) > 9 according to Agilent 2100 Bioanylzer assays) can be obtained from high quality RNA samples. The RIN of the processed sample is typically equal (±0.3) to the RIN of the input sample. The NucleoSpin® RNA Clean-up XS kit allows clean-up and concentration of RNA with an A260/A280 ratio generally exceeding 1.9. Due to the high RNA purity large amounts of eluates can successfully be used as template in RT-PCR without inhibition. 

Features