Spin technology based isolation of genomic DNA from forensic samples (e.g., casework samples, contact traces)

• Excellent DNA purity from all casework samples
• Uniform buffer system for single sample (NucleoSpin® DNA Forensic) analysis and for high throughput analysis (NucleoMag® DNA Forensic) 
• Conformity to ISO 18385 for doubtless DNA profiling

Principle

The new MACHEREY-NAGEL DNA Forensic series perfectly matches requirements of forensic laboratories. Use either the NucleoSpin® DNA Forensic kit with spin column technology for individual samples or the magnetic-bead based NucleoMag® DNA Forensic kit for automated nucleic acid isolation in high throughput formats (HTP). Uniform buffer chemistry for spin - or magnetic bead based technology facilitates highest flexibility for each individual case.

Choose between both techniques without the loss of quality.

Equivalent gDNA recovery at a constantly high level

DNA was isolated from various sample material simultaneously with our NucleoMag® DNA Forensic kit (dark blue bars) and NucleoSpin® DNA Forensic kit (light blue bars). The comparable results of both kits demonstrates the flexible use due to uniform buffer chemistry. Depending on your purification technology preference you can choose the optimal kit. The NucleoMag® DNA Forensic kit for high throughput (HTP) application, or the NucleoSpin®  DNA Forensic kit for manual single spin preparation.

NucleoSpin® DNA Forensic is designed for the small-scale preparation of highly pure genomic DNA from buccal swabs, small amounts of any tissue, cells or other forensic samples, for example, dried blood spots, ‘trace and touch’-samples or cigarette filters. The purified DNA can be used directly as template for PCR, or any kind of enzymatic reaction.

If sample carriers, such as buccal swabs are used, it is necessary to remove the carrier after incubation with the lysis buffer in an efficient and convenient way. Here, the NucleoSpin® Forensic Filters are recommended. The filters allow a long term incubation of lysis buffer and sample, with an easy and fast separation by centrifugation afterwards.

The kit provides reagents for the purification of up to 7 μg of pure genomic DNA from suitable samples (typical yields for DNA isolation from buccal swabs: 1–3 μg DNA). Depending on the elution volume used, concentrations of 10–30 ng/μL can be obtained.

NucleoSpin® DNA Forensic procedure

The NucleoSpin® DNA Forensic procedure is based on reversible adsorption of nucleic acids to the silica membrane in the NucleoSpin® DNA Forensic Columns under appropriate buffer conditions. Lysis is achieved by incubation of samples with Proteinase K at 56 °C. For the adjustment of binding conditions under which nucleic acids bind to the silica membrane, binding buffer is added to the lysate. After binding, the silica membrane is washed three times to remove contaminants and salts using 2 different wash buffers. Residual ethanol from previous wash steps is removed by a drying step. Finally, highly purified DNA is eluted with low-salt elution buffer and can directly be used for downstream applications.