Principle

The silica-matrix based kit for PCR clean-up without columns

The NucleoTraP®CR extraction system is especially designed for direct purification of DNA fragments from PCR assays without gel electrophoresis. DNA is reversibly adsorbed to the matrix with the aid of chaotropic agents. The NucleoTraP®CR matrix can selectively bind DNA fragments > 100 bp, while the primer and primer dimer molecules remain in solution. After two washing steps the DNA is eluted in TE buffer (10 mM Tris/HCl, 1 mM EDTA, pH 8.0) or another low-salt medium (e.g., water, pH 8.0–8.5). The eluted DNA shows a very high purity. All impurities such as enzymes, nucleotides and the primer molecules are quantitatively removed. The eluted DNA can be used directly for all kinds of cloning experiments (ligation reactions, labelling reactions). DNA purified with NucleoTraP®CR gives excellent results in sequencing experiments using radioactive or fluorescent detection methods.

Features