• Efficient removal of contaminating DNA – rDNase included for on-column digestion*
• Efficient sample homogenization and reduction of viscosity –  NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Plant system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size