Principle

Parallel isolation of RNA and DNA from undivided samples in one working procedure

Selective isolation of RNA and DNA from unique, precious samples is a demand for an increasing number of applications. Commonly, two independent preparations – one for RNA and one for DNA – are performed after splitting a sample. For certain samples, however, splitting is unfavourable if not impossible, for example biopsy samples or single individuals of small organisms (e.g., insects, worms). Only few methods exist for isolation of RNA and DNA from undivided samples after which RNA and DNA are available separately and immediately ready to use in common downstream applications. With the new patented NucleoSpin® RNA/DNA Buffer Set (in combination with the NucleoSpin® RNA II, NucleoSpin® RNA XS, NucleoSpin® miRNA, NucleoSpin® RNA Plant, or NucleoSpin® RNA/Protein kit) a methodology was developed that enables the isolation of RNA and DNA from unsplit samples in one working procedure using one silica-based spin column. DNA and RNA are sequentially eluted from the column using low salt buffer and water, respectively, thus facilitating immediate use of DNA and RNA for common downstream applications.

Features