The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P8109S     -20       Endoproteinase LysC P8109SVIAL -20 1 x 0.02 mg Not Applicable

Application Features

• Digestion of proteins for proteomic analysis by Mass Spectrometry
• Protein and peptide identification

Companion Products

• Trypsin-ultra™, Mass Spectrometry Grade
• Endoproteinase GluC
• Endoproteinase AspN
• α-Lytic Protease

1. Storage Conditions: Supplied in dry format from a Tris-HCl buffer. The solution can be stored at 4°C for several days or in single-use aliquots at –20°C for several months. Use only freshly reconstituted protease for best results.
2. LysC is active over a wide pH range, pH 5-12, depending on the application. Ammonium bicarbonate buffer may also be used in place of Tris-HCl.

1. In-gel Digestion Protocol for Endoproteinase LysC (P8109)
2. Typical Reaction Conditions for Endoproteinase LysC (P8109)

• Protease Selection Chart
• Substrate-based Ligase Selection Chart

1. What buffer conditions can Endoproteinase LysC be used in? Are there any special applications for which LysC is especially good?
2. How much Endoproteinase LysC do I really need to digest my protein?
3. Can Endoproteinase LysC used in a double digest with Trypsin-ultra?
4. Can Endoproteinase LysC be used in acidic buffer conditions?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P8109S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P8109S_v1_0011609
• P8109S_v1_0031705
• P8109S_v1_0041712
• P8109S_v1_10014121
• P8109S_v1_10025983
• P8109S_v1_10041433
• P8109S_v1_10048434
• P8109S_v1_10052275
• P8109S_v1_10061377
• P8109S_v1_10057639
• P8109S_v1_10067917
• P8109S_v1_10070350
• P8109S_v1_10077390
• P8109S_v1_10089255
• P8109S_v1_10093448
• P8109S_v1_10096766
• P8109S_v1_10106732
• P8109S_v1_10118357
• P8109S_v1_10122078
• P8109S_v2_10139353
• P8109S_v2_10161536
• P8109S_v2_10182823
• P8109S_v2_10196362
• P8109S_v2_10215177
• P8109S_v2_10235411
• P8109S_v2_10245213
• P8109S_v2_10257706
• P8109S_v2_10269447
• P8109S_v2_10282415

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Endoproteinase LysC

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.