Product Class: Kit

Magnetic mRNA Isolation Kit

Product Introduction

  • Designed to isolate intact poly(A)+ RNA from cells and tissue
  • Automation friendly
  • Reusable up to 3 times
Catalog # Size Concentration
S1550S 25.0 isolations

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Product Information

Description

The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)25 Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand cDNA reaction.

Figure 1: Figure 1

Consistency and wide isolation range are demonstrated by poly(A)+ RNA isolation from duplicate samples of decreasing numbers of HEPG2 cells (5 x 105 to 1 x 103) by direct lysis/binding in microtiter plate followed by mRNA isolation with the magnetic method. 1/10th of isolated mRNA is converted to oligo (dT) primed cDNA using ProtoScript First Strand cDNA Synthesis Kit (NEB #E6500) and qPCR done with validated primers for the peptidylpropyl isomerase, a low-abundance housekeeping gene.

Figure 2: Figure 2

Facilitate the isolation of poly(A)+ RNA using the Magnetic mRNA Isolation kit
This product is related to the following categories:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • S1550S     4    
      Oligo d(T)25 Magnetic Beads S1419SVIAL 4 1 x 25 mg 5 mg/ml
      Lysis/Binding Buffer B1556AVIAL 4 1 x 40 ml 1 X
      Wash Buffer I B1558AVIAL 4 1 x 40 ml 1 X
      Wash Buffer II B1559AVIAL 4 1 x 40 ml 1 X
      Low Salt Buffer B1557AVIAL 4 1 x 30 ml 1 X
      Elution Buffer B1561AVIAL 4 1 x 5 ml 1 X

Properties & Usage

Features

  • Suitable for automated high-throughput applications 
  • Eliminates need for phenol or other organic solvents 
  • No need to precipitate poly(A)+ transcripts in eluent 
  • Obtain intact poly(A)+ RNA in less than one hour 
  • Negligible gDNA contamination 
  • Reusable beads

References

  1. Wu, N. et al. (2004). Mol. Biotech.. 27, 119-125.

Protocols, Manuals & Usage

Protocols

  1. Isolation of mRNA from Mammalian Cells Using the Magnetic mRNA Isolation Kit (NEB #S1550)
  2. Isolation of mRNA from Tissue Using the Magnetic mRNA Isolation Kit (NEB #S1550)
  3. Quantitation of Isolated Poly(A) RNA from the Magnetic mRNA Isolation Kit (NEB #S1550)
  4. Protocol for Regeneration of Oligo d(T)25 beads

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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