Product Class: Other

Oligo d(T)25 Magnetic Beads

Product Introduction

Oligo d(T)25 is covalently coupled to 1 μm superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the small-scale isolation of mRNA from crude cell lysates and tissue. The isolation occurs through the hybridization of covalently coupled oligo d(T)25 to the poly(A) region present in most eukaryotic mRNAs. 

• Allows for direct mRNA isolation following lysis and second-round purification of previously isolated total RNA
• The magnetic separation technology is scalable and permits elution of intact mRNA in small volumes, thereby eliminating the need for precipitation of the isolated mRNA
• Beads can be reused up to three times 
• The isolated mRNA can either be eluted or the bound d(T)25 can be used as a primer in a first-strand cDNA reaction

Catalog # Size Concentration
S1419S 5.0 ml 5 mg/ml

Product Information

Description

Supplied as a 5 mg/ml suspension in phosphate buffered saline (PBS buffer) (pH 7.4), containing 0.05% Tween® 20 and 0.05% NaN3.

Recommended Buffers

Lysis/Binding Buffer: 100 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.5% Lithium Dodecyl Sulfate (LiDS), 1 mM EDTA, 5 mM DTT

Wash Buffer I: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.1% LiDS, 1 mM EDTA, 5 mM DTT

Wash Buffer II: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 1 mM EDTA

Low-Salt Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM LiCl, 1 mM EDTA

Elution Buffer: 20 mM Tris-HCl, pH 7.5, 1 mM EDTA

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

This product is related to the following categories:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

0.05% Tween® 20
0.05% NaN3
1X PBS
pH 7.4 @ 25°C

Support Matrix

1 μm nonporous superparamagnetic microparticles.

Binding Capacity

1 mg of Oligo d(T)25 Beads will bind 10 µg of poly(A)+ RNA.

Protocols, Manuals & Usage

Protocols

  1. cDNA synthesis on oligo (dT)25 magnetic beads (S1419)
  2. Protocol for Regeneration of Oligo d(T)25 beads
  3. Isolation of mRNA from Mammalian Cells

Tools & Resources

Selection Charts

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In