BsmBI-v2
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0739S |
BsmBI-v2 |
200 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0739L |
BsmBI-v2 |
1000 units ( 10000 units/ml ) | - | Unavailable in your region |
BsmBI-v2
This product replaces BsmBI (NEB #R0580). Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).
Catalog #R0739
Product Introduction
- Time-Saver™ qualified for digestion in 5-15 minutes
- Used in Golden Gate Assembly
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- Supplied with 1 vial of Gel Loading Dye, Purple (6X) (NEB #B7024)
- NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
- Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly
- Restriction Enzyme Cut Site: CGTCTC
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Product Source
An E. coli strain that carries the modified cloned BsmBI gene from Bacillus stearothermophilus B61. BsmBI-v2 is an engineered and improved version of BsmBI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)- This product is related to the following categories:
- Restriction Endonucleases B Products,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- DNA Preparation,
- High-throughput cloning and automation solutions,
- DNA Analysis,
- NEBridge® Golden Gate Assembly ,
- Restriction Enzyme Digestion, Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||||||||
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 55°C in a total reaction volume of 50 µl.Reaction Conditions
1X NEBuffer™ r3.1
Incubate at 55°C
1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Usage Concentration
1XActivity in NEBuffers
NEBuffer™ r1.1: <10%NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 25%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
80°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked
Activity at Temperature
@37°C: 10%Related Products
Companion Products
Materials Sold Separately
Product Notes
- BsmBI-v2 is an isoschizomer of Esp3I
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Cleavage of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Time-Saver™ Qualified Enzymes
- Type IIS Restriction Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Why is there a BsmBI site in LITMUS 38 but not LITMUS 39?
- Which restriction enzymes are used in Golden Gate Assembly?
- Which restriction enzymes are used in GoldenBraid Assembly?
- When is star activity a concern?
- Is Gel Loading Dye, Purple (6X) or Gel Loading Dye, Purple (6X), no SDS compatible with other DNA binding dyes such as SYBR® and GelRed™ during gel electrophoresis?
- How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
- What are the differences between BsmBI-v2 and its isoschizomer, Esp3I?
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0739L_v1_10059568
- R0739S_v1_10059567
- R0739S_v1_10069104
- R0739S_v1_10072879
- R0739L_v1_10072878
- R0739L_v1_10076651
- R0739S_v1_10077760
- R0739L_v1_10078100
- R0739L_v1_10079772
- R0739S_v1_10079011
- R0739S_v1_10080281
- R0739L_v1_10081461
- R0739L_v1_10081486
- R0739S_v1_10081485
- R0739S_v1_10084573
- R0739S_v1_10088076
- R0739L_v1_10088077
- R0739S_v1_10091130
- R0739S_v1_10093310
- R0739L_v1_10095228
- R0739L_v1_10097393
- R0739S_v1_10097293
- R0739L_v1_10099764
- R0739S_v1_10099762
- R0739S_v1_10105217
- R0739L_v1_10107035
- R0739S_v1_10111530
- R0739L_v1_10114601
- R0739S_v1_10118457
- R0739L_v1_10119854
- R0739S_v1_10123721
- R0739L_v1_10128738
- R0739S_v1_10131499
- R0739L_v1_10133500
- R0739S_v1_10137513
- R0739L_v1_10139502
- R0739L_v1_10139898
- R0739S_v1_10139896
- R0739S_v1_10147202
- R0739L_v1_10149877
- R0739S_v1_10151161
- R0739S_v1_10156008
- R0739L_v1_10154043
- R0739L_v1_10160776
- R0739S_v1_10160208
- R0739L_v1_10165084
- R0739S_v1_10165083
- R0739S_v1_10170459
- R0739L_v1_10170457
- R0739S_v1_10173614
- R0739L_v1_10176972
- R0739S_v1_10180154
- R0739L_v1_10180155
- R0739S_v1_10185798
- R0739L_v1_10190710
- R0739S_v1_10195335
- R0739L_v1_10202749
- R0739S_v1_10205254
- R0739L_v1_10207274
- R0739L_v1_10213962
- R0739S_v1_10212877
- R0739S_v1_10221471
- R0739L_v1_10226010
- R0739S_v1_10230698
- R0739S_v1_10231498
- R0739L_v1_10232917
- R0739L_v1_10241227
- R0739L_v1_10247626
- R0739S_v1_10243995
- R0739S_v1_10254979
- R0739L_v1_10259561
- R0739S_v1_10259560
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.BsmBI-v2
NEBuffer™ r3.1
Gel Loading Dye, Purple (6X)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.