Esp3I
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0734S |
Esp3I |
300 units ( 10000 units/ml ) | - | Unavailable in your region | |
R0734L |
Esp3I |
1500 units ( 10000 units/ml ) | - | Unavailable in your region |
Esp3I
Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).
Product Introduction
Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.
- Time-Saver™ qualified for digestion in 5-15 minutes
- Isoschizomer of BsmBI-v2
- Used in Golden Gate Assembly
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
- NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
- Restriction Enzyme Cut Site: CGTCTC(1/5)
- Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly
Catalog # | Size | Concentration |
---|---|---|
R0734S | 300.0 units | 10000 units/ml |
R0734L | 1500.0 units | 10000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Product Source
An E. coli strain that carries the cloned Esp3I gene from Erwinia species RFL3 (A. Janulaitis).- This product is related to the following categories:
- Restriction Endonucleases C G Products,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- High-throughput cloning and automation solutions,
- NEBridge® Golden Gate Assembly ,
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 37°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 100%NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: <10%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked
Related Products
Companion Products
- r3733-bsaI-hf-v2
- NEBridge® Golden Gate Assembly Kit (BsaI-HF®v2)
- BbsI-HF®
- Nuclease-free Water
- BsmBI-v2
Materials Sold Separately
Product Notes
- Esp3I is an isoschizomer of BsmBI-v2
- This enzyme is blocked by Cpg methylation. More information can be found at "Dam-Dcm and CpG Methylation".
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Expanded “assembly standards” for MoClo, GoldenBraid2.0 and other modular Golden Gate Assembly methods
- General Molecular Biology Data
- Getting Started with Molecular Cloning: Simple Tips to Improve your Cloning Efficiency
- Heat Inactivation
- Making Unmethylated (Dam- Dcm-) DNA
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction Enzymes for Droplet Digital PCR (ddPCR)
- Restriction enzymes requiring multi-sites for efficient cleavage
- Single Letter Codes
- Six Tips for a Perfect Gel Extraction
- Star Activity
- Technical Tips For Optimizing Golden Gate Assembly Reactions
- The Genetic Code
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Cleavage of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Time-Saver™ Qualified Enzymes
- Type IIS Restriction Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- Why is there a Esp3I site in LITMUS 38 but not LITMUS 39?
- What are the differences between BsmBI-v2 and its isoschizomer, Esp3I?
- When is star activity a concern?
- What does it mean to be Time-Saver™ qualified?
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
- Which restriction enzymes are used in Golden Gate Assembly?
- Which restriction enzymes are used in GoldenBraid Assembly?
- Is Gel Loading Dye, Purple (6X) or Gel Loading Dye, Purple (6X), no SDS compatible with other DNA binding dyes such as SYBR® and GelRed™ during gel electrophoresis?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0734S_L_v1_0041803
- R0734L_v1_10029674
- R0734S_v1_10030625
- R0734L_v1_10030911
- R0734S_v1_10042337
- R0734L_v1_10042331
- R0734L_v1_10056199
- R0734S_v1_10056198
- R0734S_v1_10063418
- R0734L_v1_10064299
- R0734S_v1_10069704
- R0734L_v1_10080592
- R0734L_v1_10084479
- R0734S_v1_10084481
- R0734L_v1_10091151
- R0734S_v1_10091139
- R0734S_v1_10094889
- R0734L_v1_10097985
- R0734S_v1_10097986
- R0734L_v1_10106432
- R0734S_v1_10105212
- R0734L_v1_10135927
- R0734S_v1_10135926
- R0734L_v1_10144590
- R0734S_v1_10144591
- R0734L_v1_10165274
- R0734S_v1_10165273
- R0734L_v1_10171312
- R0734S_v1_10171313
- R0734L_v1_10185077
- R0734S_v1_10185080
- R0734S_v1_10192642
- R0734L_v1_10192059
- R0734S_v1_10204861
- R0734L_v1_10204863
- R0734L_v1_10219523
- R0734S_v1_10219524
- R0734L_v1_10232767
- R0734S_v1_10248415
- R0734L_v1_10254978
- R0734L_v1_10266620
- R0734S_v1_10266621
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Esp3I
rCutSmart™ Buffer
Gel Loading Dye, Purple (6X)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.