MboI
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
R0147S |
MboI, recombinant |
500 units ( 5000 units/ml ) | - | Unavailable in your region | |
R0147L |
MboI, recombinant |
2.500 units ( 5000 units/ml ) | - | Unavailable in your region | |
R0147M |
MboI, recombinant, conc. |
2.500 units ( 25000 units/ml ) | - | Unavailable in your region |
MboI
MboI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10149199. Learn more, We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
Product Introduction
- Time-Saver™ qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
- Restriction Enzyme Cut Site: /GATC
Catalog # | Size | Concentration |
---|---|---|
R0147S | 500.0 units | 5000 units/ml |
R0147L | 2500.0 units | 5000 units/ml |
R0147M | 2500.0 units | 25000 units/ml |
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- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Product Source
An E. coli strain that carries the MboI gene from Moraxella bovis (ATCC 10900).- This product is related to the following categories:
- Restriction Endonucleases H M Products,
- Time-Saver Qualified Restriction Enzymes Products,
- This product can be used in the following applications:
- Restriction Enzyme Digestion
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 37°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 75%NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutesMethylation Sensitivity
dam methylation: Blocked
dcm methylation: Not Sensitive
CpG Methylation: Impaired by Overlapping
Isoschizomers
Related Products
Companion Products
- dam-/dcm- Competent E. coli
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
Materials Sold Separately
Product Notes
- BfuCI, DpnII and Sau3AI are isoschizomers of MboI.
- MboI cleaves to leave a 5´ GATC extension which can be efficiently ligated into BamHI, BclI, BglII, BstYI, DpnII or Sau3AI cleaved fragments.
- MboI is blocked by dam methylation, however Sau3AI is not sensitive to dam methylation.
- This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.
- Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
- Blocked by dam methylation and impaired by overlapping CpG methylation.
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Star Activity
- Traditional Cloning Quick Guide
Tools & Resources
Selection Charts
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5' Overhangs
- Time-Saver™ Qualified Enzymes
- Why Choose Recombinant Enzymes?
Web Tools
FAQs & Troubleshooting
FAQs
- What's the difference between DpnI, DpnII, MboI, and Sau3AI?
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
- Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?
Troubleshooting
Tech Tips
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0147S_L_v1_0781301
- R0147S_L_v1_0781303
- R0147S_L_v1_0781306
- R0147S_L_v1_0781311
- R0147S_L_v1_0781404
- R0147T_M_v1_0781301
- R0147T_M_v1_0781404
- R0147T_M_v1_0781410
- R0147S_L_v1_0781503
- R0147T_M_v1_0781503
- R0147T_M_v1_0781505
- R0147T_M_v1_0781507
- R0147S_L_v1_0781508
- R0147T_M_v1_0781512
- R0147S_L_v1_0781512
- R0147T_M_v1_0781605
- R0147S_L_v1_0781608
- R0147T_M_v1_0781611
- R0147T_M_v1_0791611
- R0147S_L_v1_0791707
- R0147T_M_v1_0791707
- R0147T_M_v1_0791711
- R0147S_L_v1_0801712
- R0147M_v1_10017822
- R0147L_v1_10019668
- R0147S_v1_10019669
- R0147M_v1_10035358
- R0147S_v1_10036099
- R0147L_v1_10036098
- R0147M_v1_10045003
- R0147L_v1_10051150
- R0147S_v1_10058112
- R0147L_v1_10063415
- R0147S_v1_10067176
- R0147M_v1_10064557
- R0147S_v1_10069226
- R0147L_v1_10070183
- R0147M_v1_10072401
- R0147M_v1_10084576
- R0147L_v1_10091209
- R0147M_v1_10091174
- R0147M_v1_10095394
- R0147L_v1_10095396
- R0147S_v1_10095392
- R0147L_v1_10117136
- R0147M_v1_10126468
- R0147S_v1_10126471
- R0147L_v1_10131159
- R0147M_v1_10131157
- R0147S_v1_10140683
- R0147L_v1_10147615
- R0147L_v2_10149276
- R0147M_v2_10149275
- R0147S_v2_10147618
- R0147M_v2_10171445
- R0147L_v2_10173510
- R0147S_v2_10173508
- R0147M_v2_10173318
- R0147S_v2_10201438
- R0147L_v2_10204878
- R0147L_v2_10225708
- R0147M_v2_10227065
- R0147S_v2_10230731
- R0147M_v2_10251972
- R0147L_v2_10253166
- R0147S_v2_10253167
- R0147L_v2_10272209
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.MboI
rCutSmart™ Buffer
Gel Loading Dye, Purple (6X)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.