Product Class: Other

N-Glycopeptide Binding Protein
No

Product Introduction

  • Enrich for N-glycopeptides in complex proteomics samples with this engineered and improved lectin without requiring the use of HILIC columns or cartridges.
  • Significantly improved N-glycopeptide spectra match and glycoprotein identification and discovery.
  • Broad specificity to high mannose, complex, and hybrid N-glycans, as well as fucosylated and non-fucosylated, sialylated and non-sialylated, with bi-, tri- and tetra-antennary structures.
  • Recombinant and free of protease and glycosidase activities.
Catalog # Size Concentration
P0872S 1.0 mg 1 mg/ml

Product Information

Description

N-Glycopeptide Binding Protein, also known as Fbs1-GYR lectin, is engineered to selectively bind and enrich a wide variety of N-glycopeptides in a complex proteomic sample to assist N-glycoprotein identification and discovery, without requiring the use of traditional lectin affinity chromatography (LAC) techniques, HILIC columns or cartridges. This engineered lectin, originating from human Fbs1 protein, exhibits improved specificity towards high mannose, complex, and hybrid N-glycans, as well as towards fucosylated and non-fucosylated, sialylated and non-sialylated, with bi-, tri- and tetra-antennary structures. N-Glycopeptide Binding Protein includes a SNAP-tag for immobilizing to create versatile workflows.

Product Source

An E. coli strain that carries human Fbs1 mutant gene.
This product is related to the following categories:
Glycobiology Products,
Proteome Analysis Products,
This product can be used in the following applications:
Glycobiology & Proteomics,
Glycoprotein Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

20 mM Tris-HCl
50 mM NaCl
0.2 mM TCEP
pH 8

Heat Inactivation

No

Molecular Weight

Theoretical: 45 kDa

Unit Assay Conditions

Functional Testing (N-Glycan Enrichment) - A 200 μL reaction in 20 mM Tris-HCl, 50 mM NaCl, 0.2 mM TCEP, 10% acetonitrile, pH 8.0 containing 1 nmol of fluorescently labeled sialylglycopeptide and 100 μg N-Glycopeptide Binding Protein incubated for 30 minutes at 4°C results in ≥40% enrichment as determined by UV-Vis Spectroscopy at 520 nm.

Features

  • Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
  • Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
  • Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
  • Versatile workflow

References

  1. Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
  2. Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
  3. Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.

Protocols, Manuals & Usage

Protocols

  1. N-Glycopeptide Binding Protein Typical Reaction Protocol (NEB #P8072)

FAQs & Troubleshooting

FAQs

  1. How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?
  2. What is the specificity of N-Glycopeptide Binding Protein?
  3. How strong does the N-Glycopeptide Binding Protein bind to a glycopeptide?
  4. Can N-Glycopeptide Binding Protein bind and enrich free glycans?
  5. Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
  6. What influences the binding affinity of the N-Glycopeptide Binding Protein?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.