Product Class: Other

Endoproteinase LysC
37

P8109S-cutsite

Product Introduction

Endoproteinase LysC is a serine Endoproteinase which cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.

  • Analyze complex proteomes
  • Compatible for simultaneous co-digestion with Trypsin-ultra Mass Spectrometry Grade (NEB# P8101)
  • Suitable for both in-gel and solution digests
  • Supplied in dry format
  • Optimal activity and stability for up to 12 months
Catalog # Size Concentration
P8109S 20.0 µg

Product Information

Description

Endoproteinase LysC is a serine endoproteinase, isolated from Lysobacter enzymogenes, that cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.

A 16 hour native LysC digestion of Issatchenkia orientalis Cytochrome c using a 1:50 enzyme to substrate ratio, followed by LC-MS with a Thermo™ LTQ Orbitrap XL, identified 79 peptides with 85% sequence coverage.

Product Source

Isolated from Lysobacter enzymogenes

Reconstitution

Endoproteinase LysC should be reconstituted in 200 μl double-distilled water to make a 100 ng/μl solution in 10 mM Tris-HCl, pH 8.0. Rapid autolysis is a function of enzyme concentration.
This product is related to the following categories:
Proteases Products,
Proteome Analysis Products,
This product can be used in the following applications:
Glycomics and glycoproteomics,
Protein Digestion,
Glycobiology & Proteomics,
Proteomics

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Usage Concentration

100 ng/μl

Molecular Weight

Apparent: 30000 daltons

Application Features

  • Digestion of proteins for proteomic analysis by Mass Spectrometry
  • Protein and peptide identification

Product Notes

  1. Storage Conditions: Supplied in dry format from a Tris-HCl buffer. The solution can be stored at 4°C for several days or in single-use aliquots at –20°C for several months. Use only freshly reconstituted protease for best results.
  2. LysC is active over a wide pH range, pH 5-12, depending on the application. Ammonium bicarbonate buffer may also be used in place of Tris-HCl.

Protocols, Manuals & Usage

Protocols

  1. In-gel Digestion Protocol for Endoproteinase LysC (P8109)
  2. Typical Reaction Conditions for Endoproteinase LysC (P8109)

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What buffer conditions can Endoproteinase LysC be used in? Are there any special applications for which LysC is especially good?
  2. How much Endoproteinase LysC do I really need to digest my protein?
  3. Can Endoproteinase LysC used in a double digest with Trypsin-ultra?
  4. Can Endoproteinase LysC be used in acidic buffer conditions?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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