Product Class: Other

Boletopsis grisea Lectin (BGL)
cloned at NEB recombinant 25 No

Product Introduction

  • Binds to N-glycans having at least one terminal outer arm GlcNAc as well as O-glycans containing Gal-β1,3-GalNAc-α-  within their structure.
  • Enables enrichment of GlcNAc-capped N-glycans or mucin type O-glycopeptides from complex samples in glycomics and glycoproteomics analytical workflows. 
  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
 
Catalog # Size Concentration
P0867S 1.0 ml 1 mg/ml

Product Information

Description

Boletopsis grisea Lectin (BGL) is a recombinant 15 kDa lectin from the Boletopsis grisea mushroom that has been expressed in E. coli.  BGL has two separately functioning ligand binding sites (1).  Site 1 binds to O-glycans bearing the Tn antigen (GalNAc-α-Ser/Thr) or Thomsen-Friedenreich antigen (TF-antigen; Gal-β1,3-GalNAc-α-) and Site 2 binds N-glycans with terminal GlcNAc residues.

Boletopsis grisea Lectin (BGL) binding sites



BGL has two separately functioning ligand binding sites (1). Site 1 binds to O-glycans bearing the Tn antigen (GalNAc-α-Ser/Thr) or Thomsen-Friedenreich antigen (TF-antigen; Gal-β1,3-GalNAc-α-) and Site 2 binds N-glycans with terminal GlcNAc residues.


This product is related to the following categories:
Glycobiology Products
This product can be used in the following applications:
Glycobiology & Proteomics

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

50 mM Tris-HCl
200 mM NaCl
pH 7.5 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 15 kDa

Unit Assay Conditions

100 µg BGL enriches >85% Procainamide-G0/A2 N-glycan in an Epitope Directed Glycan Enrichment (EDGE) assay (1,2).  The assay involves an enrichment protocol termed EDGE whereby 100 µg BGL lectin is incubated with 100 ng Procainamide-G0 N-glycan substrate for 1.5 hours at 25°C in 20 mM Tris-HCl pH 7.5 buffer in a total volume of 120 µl.

Product Notes

  1. BGL has only been tested for the enrichment of O-glycopeptides and free N-glycans in EDGE assays.
  2. Optimal incubation time (60 to 90 minutes) will depend on the specific substrate.
  3. A lower starting amount of BGL for the EDGE assay could be used for O-glycopeptides. This amount should be empirically determined.
  4. The formulation of BGL contains 50 mM Tris-HCl, pH 7.5 and 200 mM NaCl. If this salt concentration will affect downstream workflow (e.g., capillary electrophoresis analysis), buffer exchange treatment of BGL may be performed prior to proceeding with the enrichment reaction.

References

  1. Ganatra, M. B. et al. (2021). A bi‑specific lectin from the mushroom Boletopsis grisea and its application in glycoanalytical workflows. Sci. Rep. 11, 160.
  2. Vainauskas, S. et al. (2016). Profiling of core fucosylated N-glycans using a novel bacterial lectin that specifically recognizes α1,6 fucosylated chitobiose. Sci. Rep. 6, 34195.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Enrichment of O-glycopeptides or N-glycans with terminal GlcNAcs with Boletopsis grisea Lectin (BGL) (NEB #P0867)
  2. A Typical Epitope Directed Glycan Enrichment (EDGE) Profiling Protocol using Boletopsis grisea Lectin (BGL) (NEB #P0867)

FAQs & Troubleshooting

FAQs

  1. Can I purchase large amounts of an existing Enzyme for Innovation?
  2. What are Enzymes for Innovation?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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