β1-4 Galactosidase S
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
P0745S |
beta 1-4 Galactosidase S |
400 units ( 8000 units/ml ) | - | Unavailable in your region | |
P0745L |
beta 1-4 Galactosidase S |
2.000 units ( 8000 units/ml ) | - | Unavailable in your region |
β1-4 Galactosidase S
Product Introduction
β1-4 Galactosidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β1-4 linked galactose residues from oligosaccharides.
- Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 24 months
Catalog # | Size | Concentration |
---|---|---|
P0745S | 400.0 units | 8000 units/ml |
P0745L | 2000.0 units | 8000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
β1-4 Galactosidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-4 linked galactose residues from oligosaccharides.Specificity
Product Source
Cloned from Streptococcus pneumoniae and expressed in E. coli.- This product is related to the following categories:
- Exoglycosidases Products,
- This product can be used in the following applications:
- Expression Systems,
- Glycan Sequencing,
- Protein Digestion,
- Recombinant Glycoprotein Expression, Glycoprotein Analysis
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the terminal, β-D-galactose from 1 nmol Galβ1-4GlcNAcβ1- 3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 μl.Reaction Conditions
1X GlycoBuffer 1
Incubate at 37°C
1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)
Storage Buffer
50 mM NaCl
20 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
Heat Inactivation
65°C for 10 minutesMolecular Weight
Apparent: 231000 daltonsSpecific Activity
123,000 units/mgUnit Assay Conditions
Two fold dilutions of β1-4 Galactosidase S are incubated with 1 nmol AMClabeled substrate and 1X GlycoBuffer 1 in a 10 μl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via thin layer chromatography (1).Related Products
Companion Products
Product Notes
- Reactions may be scaled-up linearly to accommodate larger reaction volumes.
- The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1–2 μl for 1 μg of glycoprotein or 100 nM of oligosaccharide for one hour in a 10–25 μl reaction. If there is still undigested material, let the reaction go overnight.
References
- Wong-Madden, S. T. and Landry, D. (1995). Glycobiology. 5, 19-28.
Protocols, Manuals & Usage
Protocols
Application Notes
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- What are Glycosidases and their uses?
- What is the difference between β1–4 Galactosidase and β1–4 Galactosidase S?
- What is the difference between β1–4 Galactosidase S and β1–3 Galactosidase?
- What is a good positive control for β1–4 Galactosidase S?
- Can I use β1–4 Galactosidase S in a double digest with other exoglycosidases and/or endoglycosidases?
- Does this enzyme require denaturing conditions to act on glycoproteins?
- What is a good method to re-purify a glycan or glycopeptide after exoglycosidase treatment?
- Do detergents inhibit glycosidases?
- Which enzyme should be used to remove β1-4 linked Galactose residues from intact IgG?
- How much exoglycosidase should be used?
- How much exoglycosidase should be used?
Tech Tips
- A good positive control for β1-4Gal is pNP- βGal.
- Using 1-2 µl is a good starting point for a 1 hr incubation of 1µg of glycoprotein or 100 nM of oligosaccharide.
Citations & Technical Literature
Citations
Additional Citations
Publications
- Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem; May 3;88(9), 4795-802. Anal Chem.. PubMedID: 27033327
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- P0745S_L_v1_0011602
- P0745S_L_v1_0011606
- P0745S_L_v1_0011609
- P0745S_L_v1_0011706
- P0745S_L_v1_0011710
- P0745S_L_v1_0011801
- P0745S_L_v1_0011804
- P0745S_v1_10018457
- P0745L_v1_10021379
- P0745S_v1_10035007
- P0745L_v1_10035009
- P0745L_v1_10056942
- P0745S_v1_10050858
- P0745L_v1_10058808
- P0745L_v1_10061273
- P0745S_v1_10077430
- P0745L_v1_10083349
- P0745S_v1_10099645
- P0745S_v1_10124285
- P0745L_v1_10124284
- P0745S_v1_10149074
- P0745S_v1_10163654
- P0745L_v1_10163656
- P0745L_v1_10182564
- P0745S_v1_10197443
- P0745S_v1_10220428
- P0745L_v1_10226035
- P0745L_v1_10239803
- P0745S_v1_10249663
- P0745L_v1_10249665
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.β1-4 Galactosidase S
GlycoBuffer 1
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.