β1-3 Galactosidase
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
P0726S |
beta 1-3 Galactosidase |
500 units ( 10000 units/ml ) | - | Unavailable in your region |
β1-3 Galactosidase
Product Introduction
β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 linked galactose residues from oligosaccharides.
- Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 24 months
Catalog # | Size | Concentration |
---|---|---|
P0726S | 500.0 units | 10000 units/ml |
P0726L | 2500.0 units | 10000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 linked D-galactopyranosyl residues from oligosaccharides. The approximate kinetic data show > 100-fold preference for β1-3 over β1-6 linkages (1,2), and > 500-fold preference from β1-3 over β1-4 linkages (3).
Detailed Specificity: The GlcNAc(β1-6) residue is the only anomeric configuration that can effect the specificity of the enzyme enabling cleavage of the non-reducing β1-4Galactose (Fig. 1).
Product Source
Cloned from Xanthomonas manihotis and expressed in E. coli.- This product is related to the following categories:
- Exoglycosidases Products
- This product can be used in the following applications:
- Glycan Sequencing,
- Recombinant Glycoprotein Expression,
- Glycoprotein Analysis
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the terminal β-D-galactose from 1 nmol of Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.Reaction Conditions
1X GlycoBuffer 1
Supplement with 100 µg/µl Recombinant Albumin (Low-glycerol)
Incubate at 37°C
1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)
Storage Buffer
50 mM NaCl
20 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
Heat Inactivation
65°C for 10 minutesMolecular Weight
Theoretical: 66 kDaUnit Assay Conditions
Two fold dilutions of β1-3 Galactosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1, supplemented with 100 µg/ml rHSA, in a 10 µl reaction. The reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized via thin layer chromatography (1).Related Products
Companion Products
Product Notes
- Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.
References
- Wong-Madden, S.T. and Landry, D. (1995). Glycobiology. 5, 19-28.
- Guthrie, E.P. and Taron, C.H. New England Biolabs. Unpublished observation
- Monks, B. New England Biolabs. Unpublished observation
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
Application Notes
FAQs & Troubleshooting
FAQs
- How much exoglycosidase should be used?
- Do detergents inhibit exoglycosidases/endoglycosidases?
- Why have the NEB Glycosidase enzymes changed reaction buffers? What are the new reaction buffers and can I still use an enzyme with its old buffer? Where can I find the composition of the old buffers?
- What are Glycosidases and their uses?
- How much exoglycosidase should be used?
Tech Tips
Using 1-2 µl is a good starting point for a 1 hr incubation of 1µg of glycoprotein or 100 nM of oligosaccharide.
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- P0726S_L_v1_0071609
- P0726S_L_v1_0071703
- P0726S_L_v1_0071708
- P0726S_v1_10018755
- P0726L_v1_10040980
- P0726S_v1_10039249
- P0726L_v1_10052042
- P0726S_v1_10052045
- P0726S_v1_10084147
- P0726S_v1_10103796
- P0726S_v1_10118694
- P0726S_v1_10136916
- P0726S_v1_10140274
- P0726S_v1_10154357
- P0726S_v1_10185168
- P0726S_v1_10198928
- P0726S_v1_10218627
- P0726S_v1_10230895
- P0726S_v1_10255391
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.β1-3 Galactosidase
GlycoBuffer 1
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.