Product Class: Other

M13mp18 RF I DNA

Product Introduction

  • View sequence details
  • Phage vectors derived from bacteriophage M13
  • Covalently closed circular DNA
  • Includes 13 unique restriction sites, and β-galactosidase gene
  • Blue/white selection
Catalog # Size Concentration
N4018S 10.0 µg 100 µg/ml

Product Information

Description

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the β-Galactosidase gene (1). When a fragment of DNA is inserted into one of these sites, the β-Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate (2).

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source

The phage M13mp18 is propagated in E. coli ER2738(3). The replicative form of DNA is isolated from infected cells and purified by a standard plasmid purification procedure. The final preparation is tested for its suitability as a vector.
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Lac Repressor on Plasmid

0

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Product Notes

  1. M13mp18 DNA may occur in two different forms:

    1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
    2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.
    Generally single-stranded DNAs are not cut by restriction enzymes.

References

  1. Norrander, J., Kempe, T. and Messing, J. (1983). Gene. 26, 101-106.
  2. Messing, J., Crea, R. and Seeburg, P.H. (1981). Nucl. Acids Res.. 9, 309-321.
  3. Messing, J. (1979). Recombinant DNA Technical Bulletin (NIH). 2, 43-48.

Protocols, Manuals & Usage

Protocols

  1. M13mp18 Phage Transfection

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the DNA sequence of this product?

Tech Tips

M13mp18 DNA may occur in two different forms:

  1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
  2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.
Generally single-stranded DNAs are not cut by restriction enzymes.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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