Product Class: Other

Fast DNA Ladder

Product Introduction

  • For use with fast electrophoresis systems (e.g. E-Gel® and FlashGel®)
  • Size range: 50 bp to 10 kb
  • Convenient, ready-to-load format
  • Formulated with xylene cyanol dye
  • Easy to identify reference band
  • Suitable for 50 gel lanes

 

Catalog # Size Concentration
N3238S 1.0 ml 25 µg/ml

Product Information

Description

The Fast DNA ladder is a pre-mixed, ready-to-load molecular weight marker containing xylene cyanol FF dye as a tracking dye. The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for fast electrophoresis systems as well as standard electrophoresis. The digested DNA includes fragments ranging from 50 base pairs to 10 kilobases. The 1 kb fragment has increased intensity to serve as reference band. This DNA Ladder has been tested and is suitable for use with many fast electrophoresis systems, such as the FlashGel® DNA System from Lonza or the E-Gel® Precast Agarose Electrophoresis System from Invitrogen (see Product Notes below for details).

  • Recommended gel percentage range: 0.8-1.2%
  • Optimum separation on 1% 

N3238_thumb
Fast DNA Ladder. Mass values are for 0.5 μg/lane
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment Mass (ng) bp
1 36 10,002
2 36 5,001
3 36 3,001
4 38 2,000
5 28 1,500
6 108 1,000
7 43 766
8 40 500
9 33 300
10 41 150
11 61 50

Effective Size Range

50bp to 10,002bp

Storage Buffer

0.002% Xylene Cyanol
5 mM Tris-HCl
5 mM EDTA
5% Glycerol
pH 8 @ 25°C

Product Notes

  1. Preparation: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. Usage Recommendation: For standard electrophoresis applications: we recommend loading 20 µl (0.5 µg) of the Fast DNA Ladder per gel lane. The best separation occurs on a 1.2% agarose gel. Below 1%, the 50 bp fragment may not separate from the 150 bp fragment. The Fast DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    For use on fast electrophoresis systems: follow the loading recommendations of the system’s manufacturer (5 to 20 µl). The Fast DNA Ladder is suitable for use on the FlashGel® System from Lonza (1.2% gel) or on the E-Gel® system from Invitrogen. This DNA Ladder has been tested and is suitable for FlashGel® 1.2% gels, E-Gel® 0.8, 1.2 and 2% gels with ethidium bromide or SYBR dye. This DNA ladder is also suitable for the E-Gel® EX 1% and 2% gels, the E-Gel® NGS, the E-Gel® SizeSelect and the E-Gel® CloneWell gels, but may require a ½ to ¼ dilution in water for optimum results.
  3. Fast DNA Ladder is stable for at least 6 months at 25°C.
  4. For long term storage. Store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor(Ed.), Molecular Cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor Laboratory Press.

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. How much Fast DNA Ladder should I load on a gel?
  2. What percentage of agarose gel should I use?
  3. Can I store it at -20°C?
  4. Why are there extra bands visible on polyacrylamide gels?
  5. Does the Fast DNA Ladder work with the FlashGel® DNA System or the E-Gel® Precast Agarose Electrophoresis System?
  6. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  7. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

FlashGel® is a registered trademark of Lonza.
E-Gel® is a registered trademark of Life Technologies.

The purchase of this product conveys to the user the non-transferable right to use the purchased amount of product for Research Use Only. Commercial use of this product may require a license from New England Biolabs. Please contact busdev@neb.com for further details.

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