NEBNext UltraShear®
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M7634S |
NEBNext UltraShear |
24 assays | - | Unavailable in your region | |
M7634L |
NEBNext UltraShear |
96 assays | - | Unavailable in your region |
NEBNext UltraShear®
For enzymatic fragmentation and library preparation of FFPE DNA samples the NEBNext UltraShear FFPE DNA Library Prep Kit (NEB #E6655) is now available.
Catalog #M7634
Product Introduction
Enzymatic fragmentation of DNA as part of the library prep workflow provides many advantages compared to mechanical shearing. However, specialized fragmentation reagents are required for enzymatic shearing in order to maintain methylation marks on samples for methylome analysis or for use with challenging samples such as FFPE DNA.
NEBNext UltraShear® is a mix of enzymes that has been designed and optimized to fragment these sample types upstream of library preparation. This improves library quality and allows retention of methylation marks.
View or download extensive performance data in our Data Supplement.
- Compatible with methylation analysis workflows, including NEBNext® Enzymatic Methyl-seq (EM-seq™) (NEB #E7120)
- Compatible with FFPE DNA
- Fast workflow with minimal hands-on time
- For methylation analysis, improves library yields, CpG coverage and sequencing metrics
- For FFPE DNA, increases usable reads and coverage uniformity and decreases artificial mutation frequency
For enzymatic fragmentation and library preparation of FFPE DNA samples we recommend the NEBNext UltraShear FFPE DNA Library Prep Kit (NEB #E6655)
Note: For library prep with enzymatic fragmentation, starting with high-quality genomic DNA samples, we recommend NEBNext Ultra™ II FS DNA Library Prep for Illumina® (NEB #E7805, #E6177) or NEBNext UltraExpress® FS DNA Library Prep Kit (NEB #3340).
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
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Product Information
Description
View or download extensive performance data in our Data Supplement.
Enzymatic methods for DNA fragmentation in NGS workflows enable streamlined protocols, improved performance and scalability. However, specialized fragmentation reagents are required for samples for methylation analysis, to ensure that methylation marks are not removed, and for FFPE DNA. NEBNext UltraShear is a novel enzyme mix designed for fragmentation of these sample types that has a fast workflow and improves library preparation and sequencing metrics for DNA methylation studies and FFPE DNA.
NEBNext UltraShear is compatible with NEBNext Enzymatic Methyl-seq (EM-seq) (NEB #E7120).
NEBNext UltraShear in FFPE DNA sequencing workflows:
- Improved usable reads
- Lower artificial mutation frequency
NEBNext UltraShear for methylated DNA sequencing e.g., EM-seq
- Higher library yields
- Improved sequencing metrics
- Improved CpG Coverage
Note: For library prep with enzymatic fragmentation, starting with high-quality genomic DNA samples, we recommend NEBNext Ultra™ II FS DNA Library Prep for Illumina® (NEB #E7805, #E6177) or NEBNext UltraExpress® FS DNA Library Prep Kit (NEB #3340).
- This product is related to the following categories:
- FFPE DNA Products,
- DNA Fragmentation & RNA Fragmentation Products,
- Next Generation Sequencing Library Preparation Products,
Kit Components
Kit Components
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Materials Required but not Supplied
- 1X TE (10 mM Tris pH 8.0, 1 mM EDTA)
- 0.2 ml thin wall PCR tubes
- Magnetic rack/stand (NEB #S1515S; Alpaqua® #A001322 or equivalent)
- PCR machine
- Vortex
- Microcentrifuge
- Bioanalyzer®, TapeStation® or other fragment analyzer and associated consumables
- 80% Ethanol
- SPRIselect™ Reagent Kit (Beckman Coulter, Inc. #B23317), AMPure® XP Beads (Beckman Coulter, Inc. #A63881) or Monarch®® PCR & DNA Cleanup Kit (NEB# T1030S/L) are recommended for Section 1.
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546S/L) for Section 2.
- Recommended Material Not Included: NEBNext Ultra II Ligation Module (NEB #E7595) and NEBNext Multiplex Oligos (www.neb.com/oligos).
- NEBNext Enzymatic Methyl-seq Kit (NEB #E7120S/L) for Section 3.
- Formamide (Sigma #F9037-100 ml) or optional 0.1 N NaOH. Formamide is preferred. If using NaOH, please see NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) FAQs
- Nuclease-free
Related Products
Companion Products
Protocols, Manuals & Usage
Protocols
Manuals
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- Is NEBNext UltraShear™ the same as NEBNext® Ultra™ II FS or NEBNext dsDNA Fragmentase®?
- Do you really need to vortex NEBNext UltraShear™?
- For EM-seq™ workflows, what are the recommendations for fragmenting already-fragmented DNA, low integrity DNA and/or FFPE DNA with NEBNext UltraShear™?
- Following the fragmentation step of the NEBNext UltraShear™ protocol, can the reactions be stored at -20˚C?
- Do you recommend NEBNext UltraShear™ for high-sensitivity, low error rate DNA library preparation?
- Can I fragment my gDNA sample with NEBNext UltraShear® in buffers other than 1X TE (10mM Tris pH 8.0, 1mM EDTA)?
- If the fragmentation at 37˚C takes a long time (30 minutes or more), is there a way to speed up my NEBNext UltraShear fragmentation?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M7634G_v0_10197094
- M7634G_v1_10176148
- M7634L_v1_10176156
- M7634S_v1_10176152
- M7634L_v1_10212233
- M7634S_v1_10212291
- M7634G_v1_10199821
- M7634S_v1_10199825
- M7634L_v1_10199829
- M7634L_v1_10226490
- M7634S_v1_10226496
- M7634L_v1_10240168
- M7634L_v1_10240619
- M7634G_v1_10240611
- M7634S_v1_10240165
- M7634S_v1_10240615
- M7634L_v1_10248777
- M7634G_v1_10226480
- M7634L_v1_10266517
- M7634S_v1_10255254
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.NEBNext UltraShear®
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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The supporting documents available for this product can be downloaded below.