Product Class: Other

TelN Protelomerase
NEBU 30 75 Heat

GMP-grade reagent also available. Learn more.

Product Introduction

  • Isolated from phage N15
  • Cuts dsDNA at a TelN recognition sequence (56 bp) 
  • Leaves covalently closed ends at the site of cleavage
  • This is an Enzyme for Innovation (EFI) graduate.  EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications.
Catalog # Size Concentration
M0651S 250.0 units 5000 units/ml

Product Information

Description

TelN Protelomerase, from phage N15, cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.


Sequence

Product Source

An E. coli strain that carries a plasmid with the TelN gene from phage N15.
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to cleave 0.5 μg of pMiniT-TelN BsaI-linearized control plasmid (313 fmol of TelN recognition sites) in a total reaction volume of 50 μl in 30 minutes at 30°C in 1X ThermoPol Reaction Buffer.

Reaction Conditions

1X ThermoPol® Reaction Buffer
Incubate at 30°C

1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Heat Inactivation

75°C for 5 minutes

Protocols, Manuals & Usage

Protocols

  1. Protocol for use with TelN Protelomerase (M0651)

FAQs & Troubleshooting

FAQs

  1. What is the structure at the cleavage site of the dsDNA after TelN Protelomerase digestion?
  2. Does TelN Protelomerase digest TelN recognition sequences in supercoiled dsDNA (e.g. plasmid) and/or linear dsDNA?
  3. What is the activity of TelN Protelomerase in common buffers?
  4. Can I purchase large amounts of an existing Enzyme for Innovation?
  5. What are Enzymes for Innovation?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In