Thermolabile Exonuclease I
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M0568S |
Thermolabile Exonuclease I |
3000 units ( 20000 units/ml ) | - | Unavailable in your region | |
M0568L |
Thermolabile Exonuclease I |
15000 units ( 20000 units/ml ) | - | Unavailable in your region |
Thermolabile Exonuclease I
Interested in a 5-minute protocol? Try out the Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050)
Product Introduction
- DNA specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction
- Unlike Exonuclease I (NEB #M0293), Thermolabile Exonuclease I can be quickly heat inactivated at 80°C in 1 minute
- Need help finding the right exonuclease for your experiments? Try Exo Selector
Thermolabile Exonuclease I is ideal for:
- Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
- Removal of single-stranded primers for nested PCR reactions
- Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
Catalog # | Size | Concentration |
---|---|---|
M0568S | 3000.0 units | 20000 units/ml |
M0568L | 15000.0 units | 20000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3′ to 5′ direction.
Product Source
An E. coli strain that carries a cloned Thermolabile Exonuclease I gene originating from a psychrophilic marine bacterium.- This product is related to the following categories:
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit of Thermolabile Exonuclease I is defined as the amount of enzyme that will catalyze the release of 2 nmol of acid-soluble nucleotide in a total reaction volume of 100 μl in 6 minutes at 37°C in 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2 and 100 μg/ml BSA with 0.17 mg/ml single-stranded [3H]-E. coli DNA.Reaction Conditions
1X NEBuffer™ r3.1
Incubate at 37°C
1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Storage Buffer
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
0.25 M NaCl
50% Glycerol
200 µg/ml BSA
pH 7.4 @ 25°C
Heat Inactivation
80°C for 1 minuteUnit Assay Conditions
50 mM Tris-HCl (pH 7.9), 10 mM MgCl2, 100 mM NaCl, 100 μg/ml BSA and 0.17 mg/ml single-stranded [3H]-E.coli DNA.Application Features
- Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
- Removal of single-stranded primers for nested PCR reactions
- Removal of single-stranded DNA from dsDNA
Related Products
Companion Products
Materials Sold Separately
Product Notes
- This enzyme can be heat inactivated at 80°C for 1 minute, 65°C for 5 minutes or 60°C for 15 minutes.
- Thermolabile ExoI is active in the presence of 25 mM to 200 mM NaCl.
- If the amount of ssDNA to be degraded needs to be increased, we recommend adding more enzyme instead of extending the reaction time.
Protocols, Manuals & Usage
Protocols
Tools & Resources
Selection Charts
Web Tools
Research Posters
FAQs & Troubleshooting
FAQs
- How can I remove the remaining primers and dNTPs from a PCR reaction prior to DNA sequencing?
- Can Thermolabile Exonuclease I remove primers in nested PCR reactions?
- Will Thermolabile Exonuclease I degrade RNA?
- Can Thermolabile Exonuclease I be used to blunt dsDNA?
- Can Thermolabile Exonuclease I be heat inactivated?
- Can Thermolabile Exonuclease I be used with a double-stranded exonuclease to clean up plasmid preparations?
- Will Thermolabile Exonuclease I work in other buffers?
- Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M0568L_v1_10026048
- M0568S_v1_10026043
- M0568L_v1_10053281
- M0568S_v1_10053629
- M0568L_v1_10065815
- M0568S_v1_10070709
- M0568L_v1_10070708
- M0568S_v1_10080156
- M0568S_v1_10091217
- M0568L_v1_10094770
- M0568S_v1_10091853
- M0568S_v1_10101721
- M0568L_v1_10106693
- M0568S_v1_10110156
- M0568L_v1_10132865
- M0568S_v1_10132867
- M0568S_v1_10159873
- M0568L_v1_10159872
- M0568L_v1_10173715
- M0568S_v1_10178087
- M0568L_v1_10204329
- M0568S_v1_10211060
- M0568S_v1_10223092
- M0568L_v1_10225934
- M0568S_v1_10232084
- M0568S_v1_10240970
- M0568S_v1_10247567
- M0568L_v1_10246680
- M0568L_v1_10254555
- M0568S_v1_10254556
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Thermolabile Exonuclease I
NEBuffer™ r3.1
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.