Exonuclease I (E. coli)
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M0293S |
Exonuclease I |
3.000 units ( 20000 units/ml ) | - | Unavailable in your region | |
M0293L |
Exonuclease I |
15.000 units ( 20000 units/ml ) | - | Unavailable in your region |
Exonuclease I (E. coli)
A thermolabile version is also available, Thermolabile Exonuclease I (NEB #M0568)
Product Introduction
- DNA specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction
- Need help finding the right exonuclease for your experiments? Try Exo Selector
Exonuclease I is ideal for:
- Removal of single-stranded primers in PCR reactions prior to Sanger DNA sequencing or SNP analysis
- Removal of single-stranded primers for nested PCR reactions
- Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
Note: See also Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050).
Catalog # | Size | Concentration |
---|---|---|
M0293S | 3000.0 units | 20000 units/ml |
M0293L | 15000.0 units | 20000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Highlights
- Isolated from a recombinant source
- 3' → 5' single strand exonuclease
- Supplied with 10X Reaction Buffer
Product Source
An E. coli strain that carries the cloned Exo I gene from E. coli NM554.- This product is related to the following categories:
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||||||||||||||||
|
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [3H]-DNA.Reaction Conditions
1X Exonuclease I Reaction Buffer
Incubate at 37°C
1X Exonuclease I Reaction Buffer
67 mM Glycine-KOH
6.7 mM MgCl2
10 mM β-ME
(pH 9.5 @ 25°C)
Usage Concentration
10,000 units/mlStorage Buffer
10 mM Tris-HCl
100 mM NaCl
5 mM β-ME
0.5 mM EDTA
100 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C
Heat Inactivation
80°C for 20 minutesApplication Features
- Exonuclease I degrades excess single-stranded primer oligonucleotide from a reaction mixture containing double-stranded extension products
Related Products
Companion Products
Materials Sold Separately
References
- Lehman and Nussbaum (1964). J. Biol. Chem. 239,
- Kusher, et al. (1971). Proc. Natl. Acad. Sci. USA. 68,
- Kusher et al. (1972). Proc. Natl. Acad. Sci. USA. 69, 1366.
- Goldmark and Linn (1972). J. Biol. Chem. 247,
- Rosamond et al. (1979). J. Biol. Chem. 254,
Protocols, Manuals & Usage
Protocols
Application Notes
Tools & Resources
Selection Charts
Web Tools
Research Posters
FAQs & Troubleshooting
FAQs
- Can Exonuclease I be used to remove primers from amplification reactions?
- Will Exonuclease I degrade RNA?
- Can Exonuclease I be used to blunt DNA?
- Can Exonuclease I be heat inactivated?
- Will Exonuclease I work in other buffers?
- Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?
Citations & Technical Literature
Citations
Additional Citations
- Enroth, C.H., Fehler, A.O., Poulsen, L.D., Vinther, J. (2019) Excess primer degradation by Exo I improves the preparation of 3´ cDNA ligation-based sequencing libraries. Biotechniques; 67, 110-116. DOI: 10.2144/btn-2018-0178
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M0293S_L_v1_0241803
- M0293S_L_v1_0241708
- M0293L_v1_10008049
- M0293L_v1_10012898
- M0293S_v1_10013378
- M0293L_v1_10018305
- M0293S_v1_10018304
- M0293L_v1_10027759
- M0293S_v1_10028076
- M0293L_v1_10031200
- M0293S_v1_10036302
- M0293L_v1_10036303
- M0293L_v1_10039370
- M0293L_v1_10041301
- M0293L_v1_10042543
- M0293S_v1_10043564
- M0293L_v1_10045937
- M0293S_v1_10052554
- M0293L_v1_10052839
- M0293L_v1_10057017
- M0293S_v1_10056951
- M0293L_v1_10062604
- M0293S_v1_10062605
- M0293L_v1_10066733
- M0293L_v1_10072027
- M0293S_v1_10066734
- M0293L_v1_10079370
- M0293S_v1_10081875
- M0293L_v1_10081876
- M0293L_v1_10088996
- M0293S_v1_10088997
- M0293L_v1_10092693
- M0293S_v1_10092694
- M0293L_v1_10094120
- M0293S_v1_10098682
- M0293L_v1_10102241
- M0293L_v1_10111225
- M0293S_v1_10111224
- M0293L_v1_10115567
- M0293S_v1_10115568
- M0293L_v1_10118375
- M0293L_v1_10126552
- M0293S_v1_10126553
- M0293S_v1_10134525
- M0293L_v1_10134536
- M0293L_v1_10144308
- M0293S_v1_10144306
- M0293L_v1_10149093
- M0293S_v1_10149095
- M0293L_v1_10157351
- M0293S_v1_10160730
- M0293L_v1_10164861
- M0293S_v1_10164860
- M0293L_v1_10171049
- M0293S_v1_10175726
- M0293L_v1_10176991
- M0293L_v1_10186973
- M0293S_v1_10187119
- M0293L_v1_10200385
- M0293S_v1_10204835
- M0293L_v1_10208339
- M0293L_v1_10213754
- M0293S_v1_10213755
- M0293L_v1_10218653
- M0293L_v1_10229561
- M0293S_v1_10226628
- M0293L_v1_10235238
- M0293S_v1_10235239
- M0293L_v1_10247876
- M0293L_v1_10259688
- M0293S_v1_10242282
- M0293S_v1_10262429
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Exonuclease I (E. coli)
Exonuclease I Reaction Buffer
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.