Phusion™ High-Fidelity PCR Master Mix with HF Buffer
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
50X higher fidelity compared to Taq
Robust reactions - maximal success with minimal optimization
Master mix format with HF buffer ideal for a wide range of AT/GC content
Product Information
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. All that is required is the addition of template, primers and water.
Figure 1: Fidelity of DNA polymerases Relative to Taq
Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
Figure 2: Phusion DNA Polymerase generates robust amplification even with short extension times
A 1.2 kb C. elegans genomic amplicon was analyzed using different polymerases. Reactions were set up in duplicate and visualized by microfluidic LabChip analysis. All reactions were conducted according to manufacturer’s instructions using 30 cycles and extension times of either 30 seconds or 2 minutes as indicated.
Product Source
An E. coli strain that carries the Phusion DNA Polymerase gene.
This product is related to the following categories:
Phusion has different annealing temperature requirements than most PCR enzymes. Please check out www.neb.com/tmcalculator to help you determine your optimal Phusion annealing temperature.
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Specifications & Change Notifications
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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Licenses
Phusion™ DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.
PHUSION™ and THERMO SCIENTIFIC™ are trademarks and property of Thermo Fisher Scientific.
Notice to purchaser:
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.
