Product Class: Other

Taq 5X Master Mix
cloned at NEB recombinant No pcr 5

Product Introduction

The industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • 5X master mix format for easy reaction setup - just add template DNA and primers
Catalog # Size Concentration
M0285L 500.0 reactions (50.0 µl) 5 X

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Product Information

Description

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

Taq 5X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
This product is related to the following categories:
Taq DNA Polymerase Products,
Master Mixes Products,
This product can be used in the following applications:
Multiplex PCR,
Specialty PCR,
Routine PCR,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

1X Master Mix Composition

10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
5% Glycerol
0.08% IGEPAL® CA-630
0.05% Tween® 20
0.2 mM dNTPs
25 units/ml Taq DNA Polymerase
pH 8.6 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 94000 daltons

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

No

Strand Displacement

No

Resulting Ends

  • Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

Unit Definition:

One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. 

Application Features

  • PCR
  • Primer Extension
  • Colony PCR

Product Notes

  1. Taq 5X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C
  2. Taq 5X Master Mix is also stable for six months at 4°C, so for frequent use, an aliquot may be kept at 4°C.
  3. Taq 5X Master Mix should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.

References

  1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
  4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
  5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
  8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Taq 5X Master Mix (M0285)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What ends will my PCR products have?
  2. How should I determine the appropriate annealing temperature for my reaction?
  3. What should the final primer concentration be in my PCR?
  4. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  5. What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
  6. Can Taq/OneTaq® DNA Polymerases be used for nick translation?
  7. How can I optimize my PCR when using Taq DNA Polymerase?

Troubleshooting

Tech Tips

Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas, LLC

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