Product Class: Other

phi29 DNA Polymerase
NEBU cloned at NEB recombinant 30 65 Heat ralbumin

Product Introduction

Perfect for multiple displacement amplification

  • High fidelity - highly accurate replication
  • Extreme processivity - capable of generating large fragments
  • Isothermal amplification - no need for a thermocycler
Catalog # Size Concentration
M0269S 250.0 units 10000 units/ml
M0269L 1250.0 units 10000 units/ml

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Can phi29 DNA Polymerase be used in other NEBuffers?
  2. Can phi29 DNA Polymerase be used to blunt DNA?
  3. Can phi29 DNA Polymerase be used to fill in 3' overhangs?
  4. Can phi29 DNA Polymerase be used to remove 5' overhangs?
  5. Can phi29 DNA Polymerase be heat inactivated?
  6. At what rate does phi29 DNA Polymerase add nucleotides to a primed single-stranded template?
  7. Will phi29 DNA Polymerase work in rCutSmart buffer?
  8. Are NEB DNA Polymerases supplied with dNTPs?

Tech Tips

DTT in the reaction buffer can oxidize quickly with >-20°C storage temperatures, extended storage, or multiple freeze/thaws, which can reduce amplification efficiency of phi29 DNA Polymerase.  Older buffer stocks or stocks that have been repeatedly frozen and thawed should be supplemented with 4 mM DTT to obtain maximal activity.

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